This R Markdown (RMD) file performs exploratory data analysis (EDA) and differential expression (DE) analysis for the 2025 Lin manuscript.
Script Timing Start
ptm <- proc.time()
# adjusted pvalue
qval <- 0.05
pval <- 0.05
lfc <- .5
# Use max of 4 cores if available.
ncore <- ifelse((detectCores()-2)>=4,4,detectCores()-2)
Set.Seed
# This will set the random number generator
set.seed(888)
# output directory
MIB_output <- paste0(Data, "Output/MIB-DYN/")
dir.create(paste0(MIB_output))
# Graphs
MIB_graphs <- paste0(Data, "Graphs/MIB-DYN/")
dir.create(paste0(MIB_graphs))
Import protein groups data
# TMT1 txt file was regenerated from the excel. Saved as a tab delimited text file
path <- "/Data/Input/MIB-MS/Dynamic/TMT_proteinGroups"
filelist <- list.files(path=path, full.names = TRUE)
# Import protein groups. Renames by base filename using string extraction
proteinGroups_ls <- list()
for (i in seq_along(filelist)){
proteinGroups_ls[[sapply(strsplit(paste0(basename(filelist[i])), "_"), '[',1)]] <-
read.table(filelist[i], sep="\t", header = TRUE )
}
# Sort alpha numeric
mixedsort(names(proteinGroups_ls), decreasing = F)
## [1] "TMT01" "TMT02" "TMT03" "TMT04" "TMT05" "TMT06" "TMT07" "TMT08" "TMT09"
## [10] "TMT10" "TMT11" "TMT12" "TMT13" "TMT14" "TMT15" "TMT16" "TMT17" "TMT18"
## [19] "TMT19" "TMT20" "TMT21" "TMT22" "TMT23" "TMT24" "TMT25" "TMT26" "TMT27"
## [28] "TMT28" "TMT29" "TMT30" "TMT31" "TMT32" "TMT33" "TMT34" "TMT35" "TMT36"
## [37] "TMT37" "TMT38" "TMT39" "TMT40" "TMT41" "TMT42"
# Order the list of dataframe
proteinGroups_ls_ordered <- list()
for (i in seq_along(filelist)){
proteinGroups_ls_ordered[mixedsort(names(proteinGroups_ls), decreasing = F)[i]] <-
proteinGroups_ls[names(proteinGroups_ls)==mixedsort(names(proteinGroups_ls), decreasing = F)[i]]
}
# Subset to kinases only
proteinGroups_kinases_ls <- list()
for (i in seq_along(proteinGroups_ls_ordered)){
proteinGroups_kinases_ls[[names(proteinGroups_ls_ordered)[i]]] <-
subset(proteinGroups_ls_ordered[[i]], proteinGroups_ls_ordered[[i]]$Gene.names %in% c(kinases.anno$Mouse_symbol,"EGFR"))
}
# Change EGFR to hEGFR
for (i in seq_along(proteinGroups_kinases_ls)){
proteinGroups_kinases_ls[[i]]$Gene.names <-
replace(proteinGroups_kinases_ls[[i]]$Gene.name,proteinGroups_kinases_ls[[i]]$Gene.name %in% c("EGFR"),"hEGFR")
}
# Assign genes as rownames
for ( i in seq_along(proteinGroups_kinases_ls)){
rownames(proteinGroups_kinases_ls[[i]])<- proteinGroups_kinases_ls[[i]]$Gene.names
}
# Extract out Reporter intensity
kinase_raw_counts <- list()
for ( i in seq_along(proteinGroups_kinases_ls)){
kinase_raw_counts[[names(proteinGroups_kinases_ls)[i]]] <-
proteinGroups_kinases_ls[[i]][,colnames(proteinGroups_kinases_ls[[i]]) %in% c("Reporter.intensity.1","Reporter.intensity.2","Reporter.intensity.3", "Reporter.intensity.4","Reporter.intensity.5","Reporter.intensity.6")]
}
# Normalization
kinase_norm <- list()
for ( i in seq_along(kinase_raw_counts)){
kinase_norm[[names(kinase_raw_counts)[i]]]<-
data.frame(colSums(kinase_raw_counts[[i]]))
colnames(kinase_norm[[names(kinase_raw_counts)[i]]])<- "sums"
}
# Extract reporter 6 %. Reporter 6 is the pooled control.
kinase_norm_per <- list()
for (i in seq_along(kinase_raw_counts)){
kinase_norm_per[[names(kinase_raw_counts)[i]]] <-
as.data.frame(colSums(kinase_raw_counts[[i]])[6]/sum(colSums(kinase_raw_counts[[i]]))*100)
colnames(kinase_norm_per[[names(kinase_raw_counts)[i]]]) <- names(kinase_raw_counts)[[i]]
}
# Convert to matrix
kinase_norm_per_matrix <- kinase_norm_per %>% map( ~ .x %>%
rownames_to_column('Reporter.intensity.6')) %>%
reduce(full_join, by = 'Reporter.intensity.6')
# Remove column label
kinase_norm_per_matrix <- kinase_norm_per_matrix[,-which(names(kinase_norm_per_matrix) %in% c("Reporter.intensity.6"))]
# Total sum of Reporter.intensity.6 %
sum(kinase_norm_per_matrix[1,])/(length(kinase_norm_per_matrix[1,]))
## [1] 5.473136
# Get a normalization factor for each TMT experiment
kinase_norm_per_matrix["norm_factor",] <- (sum(kinase_norm_per_matrix[1,])/(length(kinase_norm_per_matrix[1,])))/kinase_norm_per_matrix[1,]
colSums(kinase_raw_counts[[1]])
## Reporter.intensity.1 Reporter.intensity.2 Reporter.intensity.3
## 35293260 37823850 31281229
## Reporter.intensity.4 Reporter.intensity.5 Reporter.intensity.6
## 33213317 38442283 11164385
sum(colSums(kinase_raw_counts[[1]]))
## [1] 187218323
colSums(kinase_raw_counts[[1]])[6]/sum(colSums(kinase_raw_counts[[1]]))
## Reporter.intensity.6
## 0.05963297
colSums(kinase_raw_counts[[1]])[1]/sum(colSums(kinase_raw_counts[[1]]))
## Reporter.intensity.1
## 0.1885139
colSums(kinase_raw_counts[[1]])[2]/sum(colSums(kinase_raw_counts[[1]]))
## Reporter.intensity.2
## 0.2020307
# Rename the columns
kinase_raw_counts_rn <- list()
for ( i in seq_along(kinase_raw_counts)){
kinase_raw_counts_rn[[names(kinase_raw_counts)[i]]] <- kinase_raw_counts[[i]]
colnames(kinase_raw_counts_rn[[names(kinase_raw_counts)[i]]]) <- c(paste0(names(kinase_raw_counts)[i],"_","R1"),
paste0(names(kinase_raw_counts)[i],"_","R2"),
paste0(names(kinase_raw_counts)[i],"_","R3"),
paste0(names(kinase_raw_counts)[i],"_","R4"),
paste0(names(kinase_raw_counts)[i],"_","R5"),
paste0(names(kinase_raw_counts)[i],"_","R6"))
}
# Extract out subset of Reporter intensity
kinase_raw_counts_subset <- list()
for ( i in seq_along(proteinGroups_kinases_ls)){
kinase_raw_counts_subset[[names(proteinGroups_kinases_ls)[i]]] <-
proteinGroups_kinases_ls[[i]][,colnames(proteinGroups_kinases_ls[[i]]) %in% c("Reporter.intensity.1","Reporter.intensity.2","Reporter.intensity.3", "Reporter.intensity.4")]
}
# Multiply all values by norm factor and round to the nearest integer
kinase_norm_intensity <- list()
for ( i in seq_along(kinase_raw_counts_subset)){
kinase_norm_intensity[[names(kinase_raw_counts_subset)[i]]] <-
round(kinase_raw_counts_subset[[i]]*kinase_norm_per_matrix["norm_factor",i])
}
# Rename the columns
kinase_norm_intensity_rn <- list()
for ( i in seq_along(kinase_norm_intensity)){
kinase_norm_intensity_rn[[names(kinase_norm_intensity)[i]]] <- kinase_norm_intensity[[i]]
colnames(kinase_norm_intensity_rn[[names(kinase_norm_intensity)[i]]]) <- c(paste0(names(kinase_norm_intensity)[i],"_","R1"),
paste0(names(kinase_norm_intensity)[i],"_","R2"),
paste0(names(kinase_norm_intensity)[i],"_","R3"),
paste0(names(kinase_norm_intensity)[i],"_","R4"))
}
# Merge list of dataframe to one big dataframe. We loop through the list with map, create a row names column with rownames_to_column and reduce to a single dataset by doing a full_join by the row names.
kinase_norm_intensity_matrix <- kinase_norm_intensity_rn %>% map( ~ .x %>%
rownames_to_column('Gene.names')) %>%
reduce(full_join, by = 'Gene.names')
# Replace all na with 0 value
kinase_norm_intensity_matrix[is.na(kinase_norm_intensity_matrix)] <- "0"
# Import master sequencing datasheet
TMT_samples_all <-
read.csv(
"/Data/Input/MIB-MS/Dynamic/240530_MIB_TMT_SampleSheet.csv",
header = TRUE,
sep = ",",
stringsAsFactors = FALSE
)
# Edit cell line name
TMT_samples_all$Cell.Line <- ifelse(TMT_samples_all$Cell.Line=="PC","CEv3",TMT_samples_all$Cell.Line)
# Convert to factors
TMT_samples_all$replicate <- as.factor(TMT_samples_all$replicate )
TMT_samples_all$Cell.Line <- as.factor(TMT_samples_all$Cell.Line )
TMT_samples_all$Timepoint <- as.factor(TMT_samples_all$Timepoint )
TMT_samples_all$clid <- as.factor(TMT_samples_all$clid)
TMT_samples_all$serum <- as.factor(TMT_samples_all$serum)
TMT_samples_all$condition <- paste0(TMT_samples_all$Cell.Line, "_" ,TMT_samples_all$Timepoint)
TMT_samples_all$condition <- as.factor(TMT_samples_all$condition)
TMT_samples_all$Prep <- as.factor(TMT_samples_all$Prep)
TMT_samples_all$replicate <- as.factor(TMT_samples_all$replicate)
TMT_samples_all$Timepoint <- as.factor(TMT_samples_all$Timepoint)
TMT_samples_all$name <- paste0(TMT_samples_all$condition, "_" ,TMT_samples_all$replicate)
TMT_samples_all$name <- as.factor(TMT_samples_all$name)
TMT_samples_all$Run <- as.factor(TMT_samples_all$Run)
TMT_samples_all$EGFR_TKI_sen <- as.factor(ifelse(TMT_samples_all$Cell.Line %in% c("E4","E5","G1","G5","G8","G12","GR1","GR7"),"Resistant","Sensitive"))
# Check the samplesheet
str(TMT_samples_all)
## 'data.frame': 168 obs. of 18 variables:
## $ Prep : Factor w/ 11 levels "Prep_01","Prep_02",..: 1 1 1 1 1 1 1 1 1 1 ...
## $ Run : Factor w/ 42 levels "TMT01","TMT02",..: 1 1 1 1 2 2 2 2 3 3 ...
## $ Number : int 1 2 3 4 6 7 8 9 11 12 ...
## $ Sample : chr "P0380" "P0381" "P0382" "P0383" ...
## $ Cell.Line : Factor w/ 14 levels "C","CE","CEv3",..: 2 2 2 2 1 1 1 1 5 5 ...
## $ clid : Factor w/ 14 levels "689","690","691",..: 14 14 14 14 13 13 13 13 12 12 ...
## $ Timepoint : Factor w/ 4 levels "0","4","24","48": 1 2 3 4 1 2 3 4 1 2 ...
## $ serum : Factor w/ 1 level "starve": 1 1 1 1 1 1 1 1 1 1 ...
## $ Harvest : int 180817 180817 180818 180819 180807 180807 180808 180809 180807 180807 ...
## $ Label_num : int 126 127 128 129 126 127 128 129 126 127 ...
## $ Tag : chr "R1" "R2" "R3" "R4" ...
## $ Exp_rep : int 3 3 3 3 3 3 3 3 3 3 ...
## $ TMT.Reporter: chr "TMT01_R1" "TMT01_R2" "TMT01_R3" "TMT01_R4" ...
## $ label : chr "TMT01_R1" "TMT01_R2" "TMT01_R3" "TMT01_R4" ...
## $ condition : Factor w/ 56 levels "C_0","C_24","C_4",..: 5 7 6 8 1 3 2 4 17 19 ...
## $ replicate : Factor w/ 3 levels "1","2","3": 3 3 3 3 3 3 3 3 3 3 ...
## $ name : Factor w/ 168 levels "C_0_1","C_0_2",..: 15 21 18 24 3 9 6 12 51 57 ...
## $ EGFR_TKI_sen: Factor w/ 2 levels "Resistant","Sensitive": 2 2 2 2 2 2 2 2 2 2 ...
# Make measure column for DEP
TMT_samples_all$measure <- "Intensity"
# Remove cell lines not part of this experiment
TMT_samples_all <- TMT_samples_all[!TMT_samples_all$Cell.Line %in% c("CEv3P", "CP", "CE", "CEv3_MA", "S1","GR1","GR7" ), ]
# Copy over adjusted intensity matrix
se_TMT_all <- kinase_norm_intensity_matrix
se_TMT_all[,2:169]<- as.numeric(unlist(se_TMT_all[,2:169]))
# Remove gene.name
row.names(se_TMT_all) <- se_TMT_all$Gene.names
se_TMT_all <- se_TMT_all[,-which(names(se_TMT_all) %in% c("Gene.names"))]
# Organize for DEP
se_TMT_all$ID <- row.names(se_TMT_all)
se_TMT_all$name <- row.names(se_TMT_all)
# Extract sample names from data matrix
cols <- grep("TMT", colnames(se_TMT_all))
# Check samples match between data matrix and sample sheet
TMT_samples_all$label %in% colnames(se_TMT_all)
## [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [16] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [31] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [46] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [61] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [76] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [91] TRUE TRUE TRUE TRUE TRUE TRUE
TMT_samples_all$label[!(TMT_samples_all$label %in% colnames(se_TMT_all))]
## character(0)
# Save sample sheet
write.csv(TMT_samples_all, file=paste0(MIB_output,"MIB_TMT_SampleSheet.csv"))
# Save Raw counts
write.csv(se_TMT_all, file=paste0(MIB_output,"MIB_TMT_RawData.csv") )
# Make DEP object
DEP_TMT <- DEP::make_se(se_TMT_all, cols, TMT_samples_all)
# Directories for DEP_Bl_Par
dir.create(paste0(MIB_graphs,"DEP_TMT_all"))
dir.create(paste0(MIB_output,"DEP_TMT_all"))
graph_dir <- paste0(MIB_graphs,"DEP_TMT_all/")
output_dir <- paste0(MIB_output,"DEP_TMT_all/")
# Copy over
se_mibs_TMT_raw <- DEP_TMT
# Build summarized experiment object
se_mibs_TMT_vsn <- DEP::normalize_vsn(se_mibs_TMT_raw)
## Warning in vsnSample(v): 34 rows were removed since they contained only NA
## elements.
# Pre and post normalization comparison
DEP::plot_normalization(se_mibs_TMT_raw,se_mibs_TMT_vsn)
# Plot a heatmap of proteins with missing values
missval_plot <- DEP::plot_missval(se_mibs_TMT_vsn)
missval_plot
# Frequency plot of missing values
freq_plot <- DEP::plot_frequency(se_mibs_TMT_vsn)
freq_plot
# Plot intensity distributions and cumulative fraction of proteins with and without missing values
detect_plot <- DEP::plot_detect(se_mibs_TMT_vsn)
## Warning: Removed 34 rows containing missing values or values outside the scale range
## (`geom_line()`).
detect_plot
## TableGrob (2 x 1) "arrange": 2 grobs
## z cells name grob
## 1 1 (1-1,1-1) arrange gtable[layout]
## 2 2 (2-2,1-1) arrange gtable[layout]
# Impute missing data using random draws from a manually defined left-shifted Gaussian distribution (for MNAR)
# scale Numeric(1), Sets the width of the distribution relative to the standard deviation of the original distribution
# shift Numeric(1), Sets the left-shift of the distribution (in standard deviations) from the median of the original distribution.
# # Begin comment out
#
# # Comment out after running once to lock in imputed values
# set.seed(888)
# se_mibs_TMT_mv <- DEP::impute(se_mibs_TMT_vsn, fun = "man", shift = 3, scale = 0.3)
#
# # write imputation data to RDS and csv to 'lock in' that specific imputation. Writing to both the impute folder and the output folder simultaneously.
#
# saveRDS(se_mibs_TMT_mv,file = paste0("/Data/Input/MIB-MS/Dynamic/","se_mibs_TMT_mv",".rds"))
# saveRDS(se_mibs_TMT_mv,file = paste0(MIB_output,"se_mibs_TMT_mv",".rds"))
#
# write.csv(assays(se_mibs_TMT_mv), paste0("/Data/Input/MIB-MS/Dynamic/","se_mibs_TMT_mv",".csv"))
# write.csv(assays(se_mibs_TMT_mv), paste0(MIB_output,"se_mibs_TMT_mv",".csv"))
#
# # End comment out
# read in imputation RDS to use for rest of code
se_mibs_TMT_mv <- readRDS(paste0("/Data/Input/MIB-MS/Dynamic/","se_mibs_TMT_mv",".rds"))
# Plot intensity distributions before and after imputation
impute_plot <- plot_imputation(se_mibs_TMT_vsn, se_mibs_TMT_mv)
impute_plot
# Batch correction using Limma
se_mibs_TMT_bc <- se_mibs_TMT_mv
assay(se_mibs_TMT_bc) <- limma::removeBatchEffect(assay(se_mibs_TMT_bc), batch = se_mibs_TMT_bc@colData$Prep , batch2=se_mibs_TMT_bc@colData$Run )
## Coefficients not estimable: batch21 batch22 batch23 batch24 batch26 batch28 batch29 batch210 batch211 batch213 batch214 batch215 batch219 batch221 batch222 batch223 batch224 batch227 batch230 batch231 batch232 batch233 batch234 batch238 batch239 batch240 batch241
## Warning: Partial NA coefficients for 306 probe(s)
boxplot(assay(se_mibs_TMT_raw), las=2, ylab="log2 ratio", main="Raw")
boxplot(assay(se_mibs_TMT_vsn), las=2, ylab="log2 ratio", main=paste0("vsn"))
boxplot(assay(se_mibs_TMT_mv), las=2, ylab="log2 ratio", main=paste0("impute missing values"))
boxplot(assay(se_mibs_TMT_bc), las=2, ylab="log2 ratio", main=paste0("batch correction"))
meanSdPlot(se_mibs_TMT_raw)
## Warning: Removed 34 rows containing non-finite outside the scale range
## (`stat_binhex()`).
## Warning: Removed 2 rows containing missing values or values outside the scale range
## (`geom_line()`).
meanSdPlot(se_mibs_TMT_vsn)
## Warning: Removed 34 rows containing non-finite outside the scale range
## (`stat_binhex()`).
## Removed 2 rows containing missing values or values outside the scale range
## (`geom_line()`).
meanSdPlot(se_mibs_TMT_mv)
meanSdPlot(se_mibs_TMT_bc)
PCA cannot be performed with missing values
# PCA with all genes
# Create pca object
pcaData <- PCAtools::pca(assay(se_mibs_TMT_mv), metadata = colData(se_mibs_TMT_mv))
# Save pca data
write.csv(pcaData$rotated, file =paste0(output_dir,"pcaData",".csv"))
# Set colors
color_vector <-
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Cell.Line',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
pairsplot
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_","pairsplot_mv", ".pdf"),
plot = pairsplot
)
## Saving 7 x 5 in image
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Cell.Line",
colkey= color_vector,
shape = "Timepoint",
legendPosition = 'right' )
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
p1
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_mv_","cell_line",".pdf"),
plot = p1
)
## Saving 7 x 5 in image
# PCA with all genes
# Create pca object
pcaData <- PCAtools::pca(assay(se_mibs_TMT_bc), metadata = colData(se_mibs_TMT_bc))
# Save pca data
write.csv(pcaData$rotated, file =paste0(output_dir,"pcaData",".csv"))
# Set colors
color_vector <-
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Cell.Line',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
pairsplot
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_","pairsplot_bc", ".pdf"),
plot = pairsplot
)
## Saving 7 x 5 in image
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Cell.Line",
colkey= color_vector,
shape = "Timepoint",
legendPosition = 'right' )
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
p1
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_bc_","cell_line",".pdf"),
plot = p1
)
## Saving 7 x 5 in image
# Directories for MIB
MIB_output
## [1] "/Data/Output/MIB-DYN/"
MIB_graphs
## [1] "/Data/Graphs/MIB-DYN/"
# Directory for full SE obj
dir.create(paste0(MIB_output,"DEP_TMT_all"))
## Warning in dir.create(paste0(MIB_output, "DEP_TMT_all")):
## '/Data/Output/MIB-DYN/DEP_TMT_all' already exists
dir.create(paste0(MIB_graphs,"DEP_TMT_all"))
## Warning in dir.create(paste0(MIB_graphs, "DEP_TMT_all")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_all' already exists
graph_dir <- paste0(MIB_graphs,"DEP_TMT_all/")
output_dir <- paste0(MIB_output,"DEP_TMT_all/")
# Directory for Full SE obj results
dir.create(paste0(output_dir,"Limma_res"))
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
graph_dir_res <- paste0(graph_dir,"Limma_res/")
se_mibs_TMT_mv@colData$condition <- as.factor(se_mibs_TMT_mv@colData$condition)
# comps
comps_factor <- as.vector(factor(paste0(colData(se_mibs_TMT_mv)$condition)))
comps_df <- crossing(comp_num=comps_factor, comp_denom=comps_factor)
comps_df <- subset(comps_df, as.character(comps_df$comp_num) != as.character(comps_df$comp_denom))
# All comps
comps_df_all <- comps_df
comps_df_all <- cbind(comp_type="condition",comps_df_all)
comps_df_all <- cbind(comp_no=1:nrow(comps_df_all),comps_df_all)
comps_df_all$comp_name <- paste(comps_df_all$comp_num, "vs", comps_df_all$comp_denom, sep = "-")
comps_df_all$comp <- paste(comps_df_all$comp_num,comps_df_all$comp_denom, sep="-")
write.csv(comps_df_all, file=paste0(output_dir_res,"comps_all.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
comps_all_subset <- comps_df_all[,2:4]
# Unique comps only (for only unique combo)
comps_df_unique <- comps_df[!duplicated(t(apply(comps_df, 1, sort))), ]
comps_df_unique <- cbind(comp_type="condition",comps_df_unique )
comps_df_unique <- cbind(comp_no=1:nrow(comps_df_unique),comps_df_unique)
comps_df_unique$comp_name <- paste(comps_df_unique$comp_num, "vs", comps_df_unique$comp_denom, sep = "-")
comps_df_unique$comp <- paste(comps_df_unique$comp_num,comps_df_unique$comp_denom, sep="-")
write.csv(comps_df_unique, file=paste0(output_dir_res,"comps_unique.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
comps_unique_subset <- comps_df_unique[,2:4]
# Comp list to use (all comps)
comps <- comps_df_all
# Extract gene matrix
gene.matrix <- as.matrix(assay(se_mibs_TMT_mv))
# make design table
cond <- as.vector(se_mibs_TMT_mv@colData$condition)
prep <- as.vector(se_mibs_TMT_mv@colData$Prep)
run <- as.vector(se_mibs_TMT_mv@colData$Run)
# Design formula with prep and run as covariates.
design <- model.matrix(~0 + cond + prep + run)
colnames(design) = gsub("cond","",colnames(design))
head(design)
## C_0 C_24 C_4 C_48 CEv3_0 CEv3_24 CEv3_4 CEv3_48 E4_0 E4_24 E4_4 E4_48 E5_0
## 1 1 0 0 0 0 0 0 0 0 0 0 0 0
## 2 0 0 1 0 0 0 0 0 0 0 0 0 0
## 3 0 1 0 0 0 0 0 0 0 0 0 0 0
## 4 0 0 0 1 0 0 0 0 0 0 0 0 0
## 5 0 0 0 0 1 0 0 0 0 0 0 0 0
## 6 0 0 0 0 0 0 1 0 0 0 0 0 0
## E5_24 E5_4 E5_48 G1_0 G1_24 G1_4 G1_48 G12_0 G12_24 G12_4 G12_48 G5_0 G5_24
## 1 0 0 0 0 0 0 0 0 0 0 0 0 0
## 2 0 0 0 0 0 0 0 0 0 0 0 0 0
## 3 0 0 0 0 0 0 0 0 0 0 0 0 0
## 4 0 0 0 0 0 0 0 0 0 0 0 0 0
## 5 0 0 0 0 0 0 0 0 0 0 0 0 0
## 6 0 0 0 0 0 0 0 0 0 0 0 0 0
## G5_4 G5_48 G8_0 G8_24 G8_4 G8_48 prepPrep_02 prepPrep_03 prepPrep_04
## 1 0 0 0 0 0 0 0 0 0
## 2 0 0 0 0 0 0 0 0 0
## 3 0 0 0 0 0 0 0 0 0
## 4 0 0 0 0 0 0 0 0 0
## 5 0 0 0 0 0 0 1 0 0
## 6 0 0 0 0 0 0 0 1 0
## prepPrep_05 prepPrep_06 prepPrep_07 prepPrep_08 prepPrep_09 prepPrep_10
## 1 0 0 0 0 0 0
## 2 0 0 0 0 0 0
## 3 0 0 0 0 0 0
## 4 0 0 0 0 0 0
## 5 0 0 0 0 0 0
## 6 0 0 0 0 0 0
## prepPrep_11 runTMT05 runTMT07 runTMT10 runTMT12 runTMT15 runTMT16 runTMT17
## 1 0 0 0 0 0 0 0 0
## 2 0 0 0 0 0 0 0 0
## 3 0 0 0 0 0 0 0 0
## 4 0 0 0 0 0 0 0 0
## 5 0 1 0 0 0 0 0 0
## 6 0 1 0 0 0 0 0 0
## runTMT18 runTMT19 runTMT20 runTMT21 runTMT25 runTMT26 runTMT27 runTMT28
## 1 0 0 0 0 0 0 0 0
## 2 0 0 0 0 0 0 0 0
## 3 0 0 0 0 0 0 0 0
## 4 0 0 0 0 0 0 0 0
## 5 0 0 0 0 0 0 0 0
## 6 0 0 0 0 0 0 0 0
## runTMT29 runTMT30 runTMT34 runTMT35 runTMT36 runTMT37 runTMT38 runTMT39
## 1 0 0 0 0 0 0 0 0
## 2 0 0 0 0 0 0 0 0
## 3 0 0 0 0 0 0 0 0
## 4 0 0 0 0 0 0 0 0
## 5 0 0 0 0 0 0 0 0
## 6 0 0 0 0 0 0 0 0
#limma part analysis
fit1 <- lmFit(gene.matrix, design)
## Coefficients not estimable: prepPrep_11 runTMT10 runTMT12 runTMT15 runTMT19 runTMT21 runTMT25 runTMT27 runTMT29 runTMT30 runTMT34 runTMT35 runTMT36 runTMT37 runTMT38 runTMT39
## Warning: Partial NA coefficients for 306 probe(s)
# Make contrasts
contrast <- makeContrasts(contrasts=comps$comp,levels=design)
fit2 <- eBayes(contrasts.fit(fit1,contrasts = contrast))
# Extract results
mibs_comp_list <- list()
for (i in seq_along(comps$comp)){
mibs_comp_list[[comps$comp[i]]] <-
topTreat(fit2, coef=comps$comp[i], number=Inf, adjust.method = "fdr")
mibs_comp_list[[comps$comp[i]]]$gene <- row.names(mibs_comp_list[[comps$comp[i]]])
}
# Estimate true null
propTrueNull(mibs_comp_list[[1]]$P.Value)
## [1] 0.8237132
# save results
for (i in seq_along(mibs_comp_list)) {
write.csv(
mibs_comp_list[[i]],
file = paste0(output_dir_res,"comp_", i, "_", names(mibs_comp_list)[i], ".csv"),
row.names = TRUE
)
}
# Results
# mibs_comp_list
# Extract results
DEP_TMT_limmaRes <- list()
for (i in seq_along(comps$comp)){
DEP_TMT_limmaRes[[comps$comp[i]]] <-
mibs_comp_list[[paste0(comps$comp[i])]]
}
# save results
for (i in seq_along(DEP_TMT_limmaRes)) {
write.csv(
DEP_TMT_limmaRes[[i]],
file = paste0(output_dir_res,"comp_", i, "_", names(DEP_TMT_limmaRes)[i], ".csv"),
row.names = TRUE
)
}
# Directories for DEP_TMT_Res
dir.create(paste0(MIB_graphs,"DEP_TMT_Res"))
dir.create(paste0(MIB_output,"DEP_TMT_Res"))
graph_dir <- paste0(MIB_graphs,"DEP_TMT_Res/")
output_dir <- paste0(MIB_output,"DEP_TMT_Res/")
# Subset
TMT_samples_all
## Prep Run Number Sample Cell.Line clid Timepoint serum Harvest
## 5 Prep_01 TMT02 6 P0298 C 701 0 starve 180807
## 6 Prep_01 TMT02 7 P0299 C 701 4 starve 180807
## 7 Prep_01 TMT02 8 P0300 C 701 24 starve 180808
## 8 Prep_01 TMT02 9 P0301 C 701 48 starve 180809
## 17 Prep_02 TMT05 21 P0294 CEv3 692 0 starve 180807
## 18 Prep_03 TMT05 22 P0295 CEv3 692 4 starve 180807
## 19 Prep_03 TMT05 23 P0296 CEv3 692 24 starve 180808
## 20 Prep_02 TMT05 24 P0297 CEv3 692 48 starve 180809
## 25 Prep_02 TMT07 31 P0117 C 701 0 starve 180705
## 26 Prep_02 TMT07 32 P0118 C 701 4 starve 180705
## 27 Prep_02 TMT07 33 P0119 C 701 24 starve 180706
## 28 Prep_02 TMT07 34 P0120 C 701 48 starve 180708
## 37 Prep_03 TMT10 46 P0290 CEv3 692 0 starve 180806
## 38 Prep_03 TMT10 47 P0291 CEv3 692 4 starve 180806
## 39 Prep_03 TMT10 48 P0292 CEv3 692 24 starve 180807
## 40 Prep_03 TMT10 49 P0293 CEv3 692 48 starve 180808
## 45 Prep_04 TMT12 56 P0113 C 701 0 starve 180704
## 46 Prep_04 TMT12 57 P0114 C 701 4 starve 180704
## 47 Prep_04 TMT12 58 P0115 C 701 24 starve 180705
## 48 Prep_04 TMT12 59 P0116 C 701 48 starve 180706
## 57 Prep_04 TMT15 71 P0286 CEv3 692 0 starve 180805
## 58 Prep_04 TMT15 72 P0287 CEv3 692 4 starve 180805
## 59 Prep_04 TMT15 73 P0288 CEv3 692 24 starve 180806
## 60 Prep_04 TMT15 74 P0289 CEv3 692 48 starve 180807
## 61 Prep_05 TMT16 76 P0368 G12 698 0 starve 180904
## 62 Prep_05 TMT16 77 P0369 G12 698 4 starve 180904
## 63 Prep_05 TMT16 78 P0370 G12 698 24 starve 180905
## 64 Prep_05 TMT16 79 P0371 G12 698 48 starve 180906
## 65 Prep_05 TMT17 81 P0356 G8 697 0 starve 180826
## 66 Prep_05 TMT17 82 P0357 G8 697 4 starve 180826
## 67 Prep_05 TMT17 83 P0358 G8 697 24 starve 180827
## 68 Prep_05 TMT17 84 P0359 G8 697 48 starve 180828
## 69 Prep_05 TMT18 86 P0344 G5 696 0 starve 180905
## 70 Prep_05 TMT18 87 P0345 G5 696 4 starve 180905
## 71 Prep_05 TMT18 88 P0346 G5 696 24 starve 180906
## 72 Prep_05 TMT18 89 P0347 G5 696 48 starve 180907
## 73 Prep_05 TMT19 91 P0332 G1 695 0 starve 180826
## 74 Prep_05 TMT19 92 P0333 G1 695 4 starve 180826
## 75 Prep_05 TMT19 93 P0334 G1 695 24 starve 180827
## 76 Prep_05 TMT19 94 P0335 G1 695 48 starve 180828
## 77 Prep_06 TMT20 96 P0204 E5 694 0 starve 180724
## 78 Prep_06 TMT20 97 P0205 E5 694 4 starve 180724
## 79 Prep_06 TMT20 98 P0206 E5 694 24 starve 180725
## 80 Prep_06 TMT20 99 P0207 E5 694 48 starve 180726
## 81 Prep_06 TMT21 101 P0189 E4 693 0 starve 180724
## 82 Prep_06 TMT21 102 P0190 E4 693 4 starve 180724
## 83 Prep_06 TMT21 103 P0191 E4 693 24 starve 180724
## 84 Prep_06 TMT21 104 P0192 E4 693 48 starve 180725
## 97 Prep_07 TMT25 121 P0364 G12 698 0 starve 180905
## 98 Prep_07 TMT25 122 P0365 G12 698 4 starve 180905
## 99 Prep_07 TMT25 123 P0366 G12 698 24 starve 180906
## 100 Prep_07 TMT25 124 P0367 G12 698 48 starve 180907
## 101 Prep_07 TMT26 126 P0352 G8 697 0 starve 180825
## 102 Prep_07 TMT26 127 P0353 G8 697 4 starve 180825
## 103 Prep_07 TMT26 128 P0354 G8 697 24 starve 180826
## 104 Prep_07 TMT26 129 P0355 G8 697 48 starve 180827
## 105 Prep_07 TMT27 131 P0340 G5 696 0 starve 180906
## 106 Prep_07 TMT27 132 P0341 G5 696 4 starve 180906
## 107 Prep_07 TMT27 133 P0342 G5 696 24 starve 180907
## 108 Prep_07 TMT27 134 P0343 G5 696 48 starve 180908
## 109 Prep_08 TMT28 136 P0328 G1 695 0 starve 180825
## 110 Prep_08 TMT28 137 P0329 G1 695 4 starve 180825
## 111 Prep_08 TMT28 138 P0330 G1 695 24 starve 180826
## 112 Prep_08 TMT28 139 P0331 G1 695 48 starve 180827
## 113 Prep_08 TMT29 141 P0200 E5 694 0 starve 180723
## 114 Prep_08 TMT29 142 P0201 E5 694 4 starve 180723
## 115 Prep_08 TMT29 143 P0202 E5 694 24 starve 180724
## 116 Prep_08 TMT29 144 P0203 E5 694 48 starve 180725
## 117 Prep_08 TMT30 146 P0185 E4 693 0 starve 180723
## 118 Prep_08 TMT30 147 P0186 E4 693 4 starve 180723
## 119 Prep_08 TMT30 148 P0187 E4 693 24 starve 180724
## 120 Prep_08 TMT30 149 P0188 E4 693 48 starve 180725
## 133 Prep_09 TMT34 166 P0360 G12 698 0 starve 180826
## 134 Prep_09 TMT34 167 P0361 G12 698 4 starve 180826
## 135 Prep_09 TMT34 168 P0362 G12 698 24 starve 180827
## 136 Prep_09 TMT34 169 P0363 G12 698 48 starve 180828
## 137 Prep_10 TMT35 171 P0348 G8 697 0 starve 180824
## 138 Prep_10 TMT35 172 P0349 G8 697 4 starve 180824
## 139 Prep_10 TMT35 173 P0350 G8 697 24 starve 180825
## 140 Prep_10 TMT35 174 P0351 G8 697 48 starve 180826
## 141 Prep_10 TMT36 176 P0336 G5 696 0 starve 180826
## 142 Prep_10 TMT36 177 P0337 G5 696 4 starve 180826
## 143 Prep_10 TMT36 178 P0338 G5 696 24 starve 180827
## 144 Prep_10 TMT36 179 P0339 G5 696 48 starve 180828
## 145 Prep_10 TMT37 181 P0324 G1 695 0 starve 180824
## 146 Prep_10 TMT37 182 P0325 G1 695 4 starve 180824
## 147 Prep_10 TMT37 183 P0326 G1 695 24 starve 180825
## 148 Prep_10 TMT37 184 P0327 G1 695 48 starve 180826
## 149 Prep_10 TMT38 186 P0196 E5 694 0 starve 180722
## 150 Prep_10 TMT38 187 P0197 E5 694 4 starve 180722
## 151 Prep_10 TMT38 188 P0198 E5 694 24 starve 180723
## 152 Prep_10 TMT38 189 P0199 E5 694 48 starve 180724
## 153 Prep_11 TMT39 191 P0181 E4 693 0 starve 180722
## 154 Prep_11 TMT39 192 P0182 E4 693 4 starve 180722
## 155 Prep_11 TMT39 193 P0183 E4 693 24 starve 180723
## 156 Prep_11 TMT39 194 P0184 E4 693 48 starve 180724
## Label_num Tag Exp_rep TMT.Reporter label condition replicate name
## 5 126 R1 3 TMT02_R1 TMT02_R1 C_0 3 C_0_3
## 6 127 R2 3 TMT02_R2 TMT02_R2 C_4 3 C_4_3
## 7 128 R3 3 TMT02_R3 TMT02_R3 C_24 3 C_24_3
## 8 129 R4 3 TMT02_R4 TMT02_R4 C_48 3 C_48_3
## 17 126 R1 3 TMT05_R1 TMT05_R1 CEv3_0 3 CEv3_0_3
## 18 127 R2 3 TMT05_R2 TMT05_R2 CEv3_4 3 CEv3_4_3
## 19 128 R3 3 TMT05_R3 TMT05_R3 CEv3_24 3 CEv3_24_3
## 20 129 R4 3 TMT05_R4 TMT05_R4 CEv3_48 3 CEv3_48_3
## 25 126 R1 2 TMT07_R1 TMT07_R1 C_0 2 C_0_2
## 26 127 R2 2 TMT07_R2 TMT07_R2 C_4 2 C_4_2
## 27 128 R3 2 TMT07_R3 TMT07_R3 C_24 2 C_24_2
## 28 129 R4 2 TMT07_R4 TMT07_R4 C_48 2 C_48_2
## 37 126 R1 2 TMT10_R1 TMT10_R1 CEv3_0 2 CEv3_0_2
## 38 127 R2 2 TMT10_R2 TMT10_R2 CEv3_4 2 CEv3_4_2
## 39 128 R3 2 TMT10_R3 TMT10_R3 CEv3_24 2 CEv3_24_2
## 40 129 R4 2 TMT10_R4 TMT10_R4 CEv3_48 2 CEv3_48_2
## 45 126 R1 1 TMT12_R1 TMT12_R1 C_0 1 C_0_1
## 46 127 R2 1 TMT12_R2 TMT12_R2 C_4 1 C_4_1
## 47 128 R3 1 TMT12_R3 TMT12_R3 C_24 1 C_24_1
## 48 129 R4 1 TMT12_R4 TMT12_R4 C_48 1 C_48_1
## 57 126 R1 1 TMT15_R1 TMT15_R1 CEv3_0 1 CEv3_0_1
## 58 127 R2 1 TMT15_R2 TMT15_R2 CEv3_4 1 CEv3_4_1
## 59 128 R3 1 TMT15_R3 TMT15_R3 CEv3_24 1 CEv3_24_1
## 60 129 R4 1 TMT15_R4 TMT15_R4 CEv3_48 1 CEv3_48_1
## 61 126 R1 3 TMT16_R1 TMT16_R1 G12_0 3 G12_0_3
## 62 127 R2 3 TMT16_R2 TMT16_R2 G12_4 3 G12_4_3
## 63 128 R3 3 TMT16_R3 TMT16_R3 G12_24 3 G12_24_3
## 64 129 R4 3 TMT16_R4 TMT16_R4 G12_48 3 G12_48_3
## 65 126 R1 3 TMT17_R1 TMT17_R1 G8_0 3 G8_0_3
## 66 127 R2 3 TMT17_R2 TMT17_R2 G8_4 3 G8_4_3
## 67 128 R3 3 TMT17_R3 TMT17_R3 G8_24 3 G8_24_3
## 68 129 R4 3 TMT17_R4 TMT17_R4 G8_48 3 G8_48_3
## 69 126 R1 3 TMT18_R1 TMT18_R1 G5_0 3 G5_0_3
## 70 127 R2 3 TMT18_R2 TMT18_R2 G5_4 3 G5_4_3
## 71 128 R3 3 TMT18_R3 TMT18_R3 G5_24 3 G5_24_3
## 72 129 R4 3 TMT18_R4 TMT18_R4 G5_48 3 G5_48_3
## 73 126 R1 3 TMT19_R1 TMT19_R1 G1_0 3 G1_0_3
## 74 127 R2 3 TMT19_R2 TMT19_R2 G1_4 3 G1_4_3
## 75 128 R3 3 TMT19_R3 TMT19_R3 G1_24 3 G1_24_3
## 76 129 R4 3 TMT19_R4 TMT19_R4 G1_48 3 G1_48_3
## 77 126 R1 3 TMT20_R1 TMT20_R1 E5_0 3 E5_0_3
## 78 127 R2 3 TMT20_R2 TMT20_R2 E5_4 3 E5_4_3
## 79 128 R3 3 TMT20_R3 TMT20_R3 E5_24 3 E5_24_3
## 80 129 R4 3 TMT20_R4 TMT20_R4 E5_48 3 E5_48_3
## 81 126 R1 3 TMT21_R1 TMT21_R1 E4_0 3 E4_0_3
## 82 127 R2 3 TMT21_R2 TMT21_R2 E4_4 3 E4_4_3
## 83 128 R3 3 TMT21_R3 TMT21_R3 E4_24 3 E4_24_3
## 84 129 R4 3 TMT21_R4 TMT21_R4 E4_48 3 E4_48_3
## 97 126 R1 2 TMT25_R1 TMT25_R1 G12_0 2 G12_0_2
## 98 127 R2 2 TMT25_R2 TMT25_R2 G12_4 2 G12_4_2
## 99 128 R3 2 TMT25_R3 TMT25_R3 G12_24 2 G12_24_2
## 100 129 R4 2 TMT25_R4 TMT25_R4 G12_48 2 G12_48_2
## 101 126 R1 2 TMT26_R1 TMT26_R1 G8_0 2 G8_0_2
## 102 127 R2 2 TMT26_R2 TMT26_R2 G8_4 2 G8_4_2
## 103 128 R3 2 TMT26_R3 TMT26_R3 G8_24 2 G8_24_2
## 104 129 R4 2 TMT26_R4 TMT26_R4 G8_48 2 G8_48_2
## 105 126 R1 2 TMT27_R1 TMT27_R1 G5_0 2 G5_0_2
## 106 127 R2 2 TMT27_R2 TMT27_R2 G5_4 2 G5_4_2
## 107 128 R3 2 TMT27_R3 TMT27_R3 G5_24 2 G5_24_2
## 108 129 R4 2 TMT27_R4 TMT27_R4 G5_48 2 G5_48_2
## 109 126 R1 2 TMT28_R1 TMT28_R1 G1_0 2 G1_0_2
## 110 127 R2 2 TMT28_R2 TMT28_R2 G1_4 2 G1_4_2
## 111 128 R3 2 TMT28_R3 TMT28_R3 G1_24 2 G1_24_2
## 112 129 R4 2 TMT28_R4 TMT28_R4 G1_48 2 G1_48_2
## 113 126 R1 2 TMT29_R1 TMT29_R1 E5_0 2 E5_0_2
## 114 127 R2 2 TMT29_R2 TMT29_R2 E5_4 2 E5_4_2
## 115 128 R3 2 TMT29_R3 TMT29_R3 E5_24 2 E5_24_2
## 116 129 R4 2 TMT29_R4 TMT29_R4 E5_48 2 E5_48_2
## 117 126 R1 2 TMT30_R1 TMT30_R1 E4_0 2 E4_0_2
## 118 127 R2 2 TMT30_R2 TMT30_R2 E4_4 2 E4_4_2
## 119 128 R3 2 TMT30_R3 TMT30_R3 E4_24 2 E4_24_2
## 120 129 R4 2 TMT30_R4 TMT30_R4 E4_48 2 E4_48_2
## 133 126 R1 1 TMT34_R1 TMT34_R1 G12_0 1 G12_0_1
## 134 127 R2 1 TMT34_R2 TMT34_R2 G12_4 1 G12_4_1
## 135 128 R3 1 TMT34_R3 TMT34_R3 G12_24 1 G12_24_1
## 136 129 R4 1 TMT34_R4 TMT34_R4 G12_48 1 G12_48_1
## 137 126 R1 1 TMT35_R1 TMT35_R1 G8_0 1 G8_0_1
## 138 127 R2 1 TMT35_R2 TMT35_R2 G8_4 1 G8_4_1
## 139 128 R3 1 TMT35_R3 TMT35_R3 G8_24 1 G8_24_1
## 140 129 R4 1 TMT35_R4 TMT35_R4 G8_48 1 G8_48_1
## 141 126 R1 1 TMT36_R1 TMT36_R1 G5_0 1 G5_0_1
## 142 127 R2 1 TMT36_R2 TMT36_R2 G5_4 1 G5_4_1
## 143 128 R3 1 TMT36_R3 TMT36_R3 G5_24 1 G5_24_1
## 144 129 R4 1 TMT36_R4 TMT36_R4 G5_48 1 G5_48_1
## 145 126 R1 1 TMT37_R1 TMT37_R1 G1_0 1 G1_0_1
## 146 127 R2 1 TMT37_R2 TMT37_R2 G1_4 1 G1_4_1
## 147 128 R3 1 TMT37_R3 TMT37_R3 G1_24 1 G1_24_1
## 148 129 R4 1 TMT37_R4 TMT37_R4 G1_48 1 G1_48_1
## 149 126 R1 1 TMT38_R1 TMT38_R1 E5_0 1 E5_0_1
## 150 127 R2 1 TMT38_R2 TMT38_R2 E5_4 1 E5_4_1
## 151 128 R3 1 TMT38_R3 TMT38_R3 E5_24 1 E5_24_1
## 152 129 R4 1 TMT38_R4 TMT38_R4 E5_48 1 E5_48_1
## 153 126 R1 1 TMT39_R1 TMT39_R1 E4_0 1 E4_0_1
## 154 127 R2 1 TMT39_R2 TMT39_R2 E4_4 1 E4_4_1
## 155 128 R3 1 TMT39_R3 TMT39_R3 E4_24 1 E4_24_1
## 156 129 R4 1 TMT39_R4 TMT39_R4 E4_48 1 E4_48_1
## EGFR_TKI_sen measure
## 5 Sensitive Intensity
## 6 Sensitive Intensity
## 7 Sensitive Intensity
## 8 Sensitive Intensity
## 17 Sensitive Intensity
## 18 Sensitive Intensity
## 19 Sensitive Intensity
## 20 Sensitive Intensity
## 25 Sensitive Intensity
## 26 Sensitive Intensity
## 27 Sensitive Intensity
## 28 Sensitive Intensity
## 37 Sensitive Intensity
## 38 Sensitive Intensity
## 39 Sensitive Intensity
## 40 Sensitive Intensity
## 45 Sensitive Intensity
## 46 Sensitive Intensity
## 47 Sensitive Intensity
## 48 Sensitive Intensity
## 57 Sensitive Intensity
## 58 Sensitive Intensity
## 59 Sensitive Intensity
## 60 Sensitive Intensity
## 61 Resistant Intensity
## 62 Resistant Intensity
## 63 Resistant Intensity
## 64 Resistant Intensity
## 65 Resistant Intensity
## 66 Resistant Intensity
## 67 Resistant Intensity
## 68 Resistant Intensity
## 69 Resistant Intensity
## 70 Resistant Intensity
## 71 Resistant Intensity
## 72 Resistant Intensity
## 73 Resistant Intensity
## 74 Resistant Intensity
## 75 Resistant Intensity
## 76 Resistant Intensity
## 77 Resistant Intensity
## 78 Resistant Intensity
## 79 Resistant Intensity
## 80 Resistant Intensity
## 81 Resistant Intensity
## 82 Resistant Intensity
## 83 Resistant Intensity
## 84 Resistant Intensity
## 97 Resistant Intensity
## 98 Resistant Intensity
## 99 Resistant Intensity
## 100 Resistant Intensity
## 101 Resistant Intensity
## 102 Resistant Intensity
## 103 Resistant Intensity
## 104 Resistant Intensity
## 105 Resistant Intensity
## 106 Resistant Intensity
## 107 Resistant Intensity
## 108 Resistant Intensity
## 109 Resistant Intensity
## 110 Resistant Intensity
## 111 Resistant Intensity
## 112 Resistant Intensity
## 113 Resistant Intensity
## 114 Resistant Intensity
## 115 Resistant Intensity
## 116 Resistant Intensity
## 117 Resistant Intensity
## 118 Resistant Intensity
## 119 Resistant Intensity
## 120 Resistant Intensity
## 133 Resistant Intensity
## 134 Resistant Intensity
## 135 Resistant Intensity
## 136 Resistant Intensity
## 137 Resistant Intensity
## 138 Resistant Intensity
## 139 Resistant Intensity
## 140 Resistant Intensity
## 141 Resistant Intensity
## 142 Resistant Intensity
## 143 Resistant Intensity
## 144 Resistant Intensity
## 145 Resistant Intensity
## 146 Resistant Intensity
## 147 Resistant Intensity
## 148 Resistant Intensity
## 149 Resistant Intensity
## 150 Resistant Intensity
## 151 Resistant Intensity
## 152 Resistant Intensity
## 153 Resistant Intensity
## 154 Resistant Intensity
## 155 Resistant Intensity
## 156 Resistant Intensity
TMT_samples_Res <- subset(TMT_samples_all,TMT_samples_all$Cell.Line %in% c("CEv3","E4","E5","G1","G5","G8","G12"))
# Subset DEP object
DEP_TMT_Res <- se_mibs_TMT_bc[,paste0(TMT_samples_Res$name)]
# Save sample sheet
write.csv(TMT_samples_Res, file=paste0(output_dir,"DEP_TMT_Res",".csv"))
# Save DEP_2019_parental as RDS
saveRDS(DEP_TMT_Res, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","DEP_TMT_Res",".rds"))
# PCA with all genes
# Create pca object
pcaData <- PCAtools::pca(assay(DEP_TMT_Res), metadata = colData(DEP_TMT_Res))
# Save pca data
write.csv(pcaData$rotated, file =paste0(output_dir,"pcaData",".csv"))
# Set colors
color_vector <-
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Cell.Line',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
pairsplot
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_","pairsplot", ".pdf"),
plot = pairsplot
)
## Saving 7 x 5 in image
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Cell.Line",
colkey= color_vector,
shape = "Timepoint",
legendPosition = 'right' )
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
p1
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_vsd_500_","cell_line",".pdf"),
plot = p1
)
## Saving 7 x 5 in image
# calculate sample euclidian distances
sampleDists <- dist(t(assay(DEP_TMT_Res)))
# plot sample euclidian distances via a heatmap
sampleDistMatrix <- as.matrix( sampleDists )
# Complex Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_TMT_Res@colData$Cell.Line,
Time = DEP_TMT_Res@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
))
)
# Heatmap of euclidian distances
ComplexHeatmap::Heatmap(
sampleDistMatrix,
name = " ",
column_title = "Eculidian Distances",
show_column_names = FALSE,
show_row_names = FALSE,
clustering_distance_rows = "euclidean",
clustering_distance_columns = "euclidean",
clustering_method_rows = "complete",
clustering_method_columns = "complete",
col = colorRampPalette( rev(brewer.pal(9, "Blues")) )(255),
top_annotation = annotation_col
)
# DEP_TMT_Res
# Directories
dir.create(paste0(output_dir,"Heatmap_TVG"))
output_dir_Heatmap_TVG <- paste0(output_dir,"Heatmap_TVG/")
dir.create(paste0(graph_dir,"Heatmap_TVG"))
graph_dir_Heatmap_TVG <- paste0(graph_dir,"Heatmap_TVG/")
# pHeatmap of Top X DEG on transformed data
tvg <- c(100,50,25,10)
topVarGenesX_list <- list()
# pHeatmap of Top X DEG on transformed data
# Extract out distances
mat_list <- list()
for (i in seq_along(tvg)) {
topVarGenesX_list[[paste0(tvg[i])]] <- head( order( rowVars( as.matrix(assay(DEP_TMT_Res)) ), decreasing=TRUE ), tvg[[i]] )
}
for (i in seq_along(tvg)) {
mat_list[[paste0(tvg[i])]] <- assay(DEP_TMT_Res)[ topVarGenesX_list[[i]], ]
}
# Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_TMT_Res@colData$Cell.Line,
Time = DEP_TMT_Res@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
))
)
# Generate Unlabeled Heatmap
p_list <- list()
for (i in seq_along(mat_list)) {
p_list[[paste0(names(mat_list)[[i]])]] <- ComplexHeatmap::Heatmap(
t(scale(t(mat_list[[i]]))),
top_annotation = annotation_col,
name = " ",
column_title = paste0(names(mat_list)[i],"_TVG"),
show_column_names = F,
show_row_names = F
)
print(p_list[[i]])
}
# Save Heatmap
for (i in seq_along(p_list)) {
pdf(file=paste0(graph_dir_Heatmap_TVG, "CHeatmap_" , names(p_list)[[i]], ".pdf"))
draw(p_list[[i]])
dev.off()
}
# Generate labeled Heatmap
p_list <- list()
for (i in seq_along(mat_list)) {
p_list[[paste0(names(mat_list)[[i]])]] <- ComplexHeatmap::Heatmap(
t(scale(t(mat_list[[i]]))),
#column_order=row.names(DEP_TMT_Res@colData[order(DEP_TMT_Res@colData$clid),]),
top_annotation = annotation_col,
column_split = factor(DEP_TMT_Res@colData$clid),
name = " ",
column_title = paste0(names(mat_list)[i],"_TVG"),
show_column_names = F,
show_row_names = T
)
print(p_list[[i]])
}
# Save Heatmap
for (i in seq_along(p_list)) {
pdf(file=paste0(graph_dir_Heatmap_TVG, "CHeatmapLabel_" , names(p_list)[[i]], ".pdf"))
draw(p_list[[i]])
dev.off()
}
# Graphics termination
if(!is.null(dev.list())) dev.off()
## null device
## 1
if(!is.null(dev.list())) graphics.off()
# Directories
dir.create(paste0(output_dir,"Limma_res"))
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# comps
comps_factor <- as.vector(factor(paste0(colData(DEP_TMT_Res)$condition)))
comps_df <- crossing(comp_num=comps_factor, comp_denom=comps_factor)
comps_df <- subset(comps_df, as.character(comps_df$comp_num) != as.character(comps_df$comp_denom))
# All comps
comps_df_all <- comps_df
comps_df_all <- cbind(comp_type="condition",comps_df_all)
comps_df_all <- cbind(comp_no=1:nrow(comps_df_all),comps_df_all)
comps_df_all$comp_name <- paste(comps_df_all$comp_num, "vs", comps_df_all$comp_denom, sep = "-")
comps_df_all$comp <- paste(comps_df_all$comp_num,comps_df_all$comp_denom, sep="-")
write.csv(comps_df_all, file=paste0(output_dir_res,"comps_all.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
comps_all_subset <- comps_df_all[,2:4]
# Unique comps only (for only unique combo)
comps_df_unique <- comps_df[!duplicated(t(apply(comps_df, 1, sort))), ]
comps_df_unique <- cbind(comp_type="condition",comps_df_unique )
comps_df_unique <- cbind(comp_no=1:nrow(comps_df_unique),comps_df_unique)
comps_df_unique$comp_name <- paste(comps_df_unique$comp_num, "vs", comps_df_unique$comp_denom, sep = "-")
comps_df_unique$comp <- paste(comps_df_unique$comp_num,comps_df_unique$comp_denom, sep="-")
write.csv(comps_df_unique, file=paste0(output_dir_res,"comps_unique.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
comps_unique_subset <- comps_df_unique[,2:4]
# Comp list to use (all comps)
comps <- comps_df_all
# Extract
# Results
# mibs_comp_list
# Directories
dir.create(paste0(output_dir,"Limma_res"))
## Warning in dir.create(paste0(output_dir, "Limma_res")):
## '/Data/Output/MIB-DYN/DEP_TMT_Res/Limma_res' already exists
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
## Warning in dir.create(paste0(graph_dir, "Limma_res")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_Res/Limma_res' already exists
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# Extract results
DEP_TMT_limmaRes <- list()
for (i in seq_along(comps$comp)){
DEP_TMT_limmaRes[[comps$comp[i]]] <-
mibs_comp_list[[paste0(comps$comp[i])]]
}
# save results
for (i in seq_along(DEP_TMT_limmaRes)) {
write.csv(
DEP_TMT_limmaRes[[i]],
file = paste0(output_dir_res,"comp_", i, "_", names(DEP_TMT_limmaRes)[i], ".csv"),
row.names = TRUE
)
}
# Directories
dir.create(paste0(output_dir,"Limma_res"))
## Warning in dir.create(paste0(output_dir, "Limma_res")):
## '/Data/Output/MIB-DYN/DEP_TMT_Res/Limma_res' already exists
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
## Warning in dir.create(paste0(graph_dir, "Limma_res")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_Res/Limma_res' already exists
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# Extract comps of interest
coi <- comps_df_all[comps_df_all$comp_denom=="CEv3_0",]
comp_list_oi <- DEP_TMT_limmaRes[names(DEP_TMT_limmaRes) %in% coi$comp]
comp_list_oi <- lapply(comp_list_oi,data.frame)
# Label list of DF
for (i in seq_along(comp_list_oi)){
colnames(comp_list_oi[[i]]) <- paste0(colnames(comp_list_oi[[i]]),"_",names(comp_list_oi)[[i]])
comp_list_oi[[i]]$Gene_ID <- row.names(comp_list_oi[[i]])
}
# Left_join comps of interest to 1x spreadsheet
comp_list_oi_master <- Reduce(function(x, y) left_join(x, y, by="Gene_ID"), comp_list_oi)
row.names(comp_list_oi_master) <- comp_list_oi_master$Gene_ID
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)=="Gene_ID")]
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)%in% paste0(grep("gene_",colnames(comp_list_oi_master), value = T)))]
# Subset to spreadsheets
comp_list_oi_padj <- comp_list_oi_master[,grep("adj.P.Val_", colnames(comp_list_oi_master))]
comp_list_oi_pvalue <- comp_list_oi_master[,grep("P.Value_", colnames(comp_list_oi_master))]
comp_list_oi_l2fc <- comp_list_oi_master[,grep("logFC_", colnames(comp_list_oi_master))]
# Save spreadsheets
write.csv(comp_list_oi_padj, file = paste0(output_dir_res,"All_coi_","padj",".csv"))
write.csv(comp_list_oi_pvalue, file = paste0(output_dir_res,"All_coi_","pvalue",".csv"))
write.csv(comp_list_oi_l2fc, file = paste0(output_dir_res,"All_coi_","l2fc",".csv"))
# Directory for volcano plots
dir.create(paste0(graph_dir,"/","volcano"))
graph_dir_vol <- paste0(graph_dir,"volcano/")
# Plot Volcano
volcano_plots_ls <- list()
for (i in seq_along(DEP_TMT_limmaRes)){
plot(volcano_plots_ls[[names(DEP_TMT_limmaRes)[i]]] <-
EnhancedVolcano(
DEP_TMT_limmaRes[[i]],
lab=row.names(DEP_TMT_limmaRes[[i]]),
x="logFC",
y="adj.P.Val",
title= paste0("MIBs ",names(DEP_TMT_limmaRes)[[i]]),
pCutoff =qval,
FCcutoff =lfc,
pointSize = 2.0,
labSize = 3.0
))}
# Save Volcano
for (i in seq_along(volcano_plots_ls)){
ggsave(filename = paste0(graph_dir_vol,"comp_", i, "_", names(volcano_plots_ls)[[i]],".pdf"), plot=volcano_plots_ls[[i]])
}
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
# Graphics termination
if(!is.null(dev.list())) dev.off()
## null device
## 1
if(!is.null(dev.list())) graphics.off()
# Directories for DEP_TMT_Par
dir.create(paste0(MIB_graphs,"DEP_TMT_Par"))
dir.create(paste0(MIB_output,"DEP_TMT_Par"))
graph_dir <- paste0(MIB_graphs,"DEP_TMT_Par/")
output_dir <- paste0(MIB_output,"DEP_TMT_Par/")
# Subset
TMT_samples_all
## Prep Run Number Sample Cell.Line clid Timepoint serum Harvest
## 5 Prep_01 TMT02 6 P0298 C 701 0 starve 180807
## 6 Prep_01 TMT02 7 P0299 C 701 4 starve 180807
## 7 Prep_01 TMT02 8 P0300 C 701 24 starve 180808
## 8 Prep_01 TMT02 9 P0301 C 701 48 starve 180809
## 17 Prep_02 TMT05 21 P0294 CEv3 692 0 starve 180807
## 18 Prep_03 TMT05 22 P0295 CEv3 692 4 starve 180807
## 19 Prep_03 TMT05 23 P0296 CEv3 692 24 starve 180808
## 20 Prep_02 TMT05 24 P0297 CEv3 692 48 starve 180809
## 25 Prep_02 TMT07 31 P0117 C 701 0 starve 180705
## 26 Prep_02 TMT07 32 P0118 C 701 4 starve 180705
## 27 Prep_02 TMT07 33 P0119 C 701 24 starve 180706
## 28 Prep_02 TMT07 34 P0120 C 701 48 starve 180708
## 37 Prep_03 TMT10 46 P0290 CEv3 692 0 starve 180806
## 38 Prep_03 TMT10 47 P0291 CEv3 692 4 starve 180806
## 39 Prep_03 TMT10 48 P0292 CEv3 692 24 starve 180807
## 40 Prep_03 TMT10 49 P0293 CEv3 692 48 starve 180808
## 45 Prep_04 TMT12 56 P0113 C 701 0 starve 180704
## 46 Prep_04 TMT12 57 P0114 C 701 4 starve 180704
## 47 Prep_04 TMT12 58 P0115 C 701 24 starve 180705
## 48 Prep_04 TMT12 59 P0116 C 701 48 starve 180706
## 57 Prep_04 TMT15 71 P0286 CEv3 692 0 starve 180805
## 58 Prep_04 TMT15 72 P0287 CEv3 692 4 starve 180805
## 59 Prep_04 TMT15 73 P0288 CEv3 692 24 starve 180806
## 60 Prep_04 TMT15 74 P0289 CEv3 692 48 starve 180807
## 61 Prep_05 TMT16 76 P0368 G12 698 0 starve 180904
## 62 Prep_05 TMT16 77 P0369 G12 698 4 starve 180904
## 63 Prep_05 TMT16 78 P0370 G12 698 24 starve 180905
## 64 Prep_05 TMT16 79 P0371 G12 698 48 starve 180906
## 65 Prep_05 TMT17 81 P0356 G8 697 0 starve 180826
## 66 Prep_05 TMT17 82 P0357 G8 697 4 starve 180826
## 67 Prep_05 TMT17 83 P0358 G8 697 24 starve 180827
## 68 Prep_05 TMT17 84 P0359 G8 697 48 starve 180828
## 69 Prep_05 TMT18 86 P0344 G5 696 0 starve 180905
## 70 Prep_05 TMT18 87 P0345 G5 696 4 starve 180905
## 71 Prep_05 TMT18 88 P0346 G5 696 24 starve 180906
## 72 Prep_05 TMT18 89 P0347 G5 696 48 starve 180907
## 73 Prep_05 TMT19 91 P0332 G1 695 0 starve 180826
## 74 Prep_05 TMT19 92 P0333 G1 695 4 starve 180826
## 75 Prep_05 TMT19 93 P0334 G1 695 24 starve 180827
## 76 Prep_05 TMT19 94 P0335 G1 695 48 starve 180828
## 77 Prep_06 TMT20 96 P0204 E5 694 0 starve 180724
## 78 Prep_06 TMT20 97 P0205 E5 694 4 starve 180724
## 79 Prep_06 TMT20 98 P0206 E5 694 24 starve 180725
## 80 Prep_06 TMT20 99 P0207 E5 694 48 starve 180726
## 81 Prep_06 TMT21 101 P0189 E4 693 0 starve 180724
## 82 Prep_06 TMT21 102 P0190 E4 693 4 starve 180724
## 83 Prep_06 TMT21 103 P0191 E4 693 24 starve 180724
## 84 Prep_06 TMT21 104 P0192 E4 693 48 starve 180725
## 97 Prep_07 TMT25 121 P0364 G12 698 0 starve 180905
## 98 Prep_07 TMT25 122 P0365 G12 698 4 starve 180905
## 99 Prep_07 TMT25 123 P0366 G12 698 24 starve 180906
## 100 Prep_07 TMT25 124 P0367 G12 698 48 starve 180907
## 101 Prep_07 TMT26 126 P0352 G8 697 0 starve 180825
## 102 Prep_07 TMT26 127 P0353 G8 697 4 starve 180825
## 103 Prep_07 TMT26 128 P0354 G8 697 24 starve 180826
## 104 Prep_07 TMT26 129 P0355 G8 697 48 starve 180827
## 105 Prep_07 TMT27 131 P0340 G5 696 0 starve 180906
## 106 Prep_07 TMT27 132 P0341 G5 696 4 starve 180906
## 107 Prep_07 TMT27 133 P0342 G5 696 24 starve 180907
## 108 Prep_07 TMT27 134 P0343 G5 696 48 starve 180908
## 109 Prep_08 TMT28 136 P0328 G1 695 0 starve 180825
## 110 Prep_08 TMT28 137 P0329 G1 695 4 starve 180825
## 111 Prep_08 TMT28 138 P0330 G1 695 24 starve 180826
## 112 Prep_08 TMT28 139 P0331 G1 695 48 starve 180827
## 113 Prep_08 TMT29 141 P0200 E5 694 0 starve 180723
## 114 Prep_08 TMT29 142 P0201 E5 694 4 starve 180723
## 115 Prep_08 TMT29 143 P0202 E5 694 24 starve 180724
## 116 Prep_08 TMT29 144 P0203 E5 694 48 starve 180725
## 117 Prep_08 TMT30 146 P0185 E4 693 0 starve 180723
## 118 Prep_08 TMT30 147 P0186 E4 693 4 starve 180723
## 119 Prep_08 TMT30 148 P0187 E4 693 24 starve 180724
## 120 Prep_08 TMT30 149 P0188 E4 693 48 starve 180725
## 133 Prep_09 TMT34 166 P0360 G12 698 0 starve 180826
## 134 Prep_09 TMT34 167 P0361 G12 698 4 starve 180826
## 135 Prep_09 TMT34 168 P0362 G12 698 24 starve 180827
## 136 Prep_09 TMT34 169 P0363 G12 698 48 starve 180828
## 137 Prep_10 TMT35 171 P0348 G8 697 0 starve 180824
## 138 Prep_10 TMT35 172 P0349 G8 697 4 starve 180824
## 139 Prep_10 TMT35 173 P0350 G8 697 24 starve 180825
## 140 Prep_10 TMT35 174 P0351 G8 697 48 starve 180826
## 141 Prep_10 TMT36 176 P0336 G5 696 0 starve 180826
## 142 Prep_10 TMT36 177 P0337 G5 696 4 starve 180826
## 143 Prep_10 TMT36 178 P0338 G5 696 24 starve 180827
## 144 Prep_10 TMT36 179 P0339 G5 696 48 starve 180828
## 145 Prep_10 TMT37 181 P0324 G1 695 0 starve 180824
## 146 Prep_10 TMT37 182 P0325 G1 695 4 starve 180824
## 147 Prep_10 TMT37 183 P0326 G1 695 24 starve 180825
## 148 Prep_10 TMT37 184 P0327 G1 695 48 starve 180826
## 149 Prep_10 TMT38 186 P0196 E5 694 0 starve 180722
## 150 Prep_10 TMT38 187 P0197 E5 694 4 starve 180722
## 151 Prep_10 TMT38 188 P0198 E5 694 24 starve 180723
## 152 Prep_10 TMT38 189 P0199 E5 694 48 starve 180724
## 153 Prep_11 TMT39 191 P0181 E4 693 0 starve 180722
## 154 Prep_11 TMT39 192 P0182 E4 693 4 starve 180722
## 155 Prep_11 TMT39 193 P0183 E4 693 24 starve 180723
## 156 Prep_11 TMT39 194 P0184 E4 693 48 starve 180724
## Label_num Tag Exp_rep TMT.Reporter label condition replicate name
## 5 126 R1 3 TMT02_R1 TMT02_R1 C_0 3 C_0_3
## 6 127 R2 3 TMT02_R2 TMT02_R2 C_4 3 C_4_3
## 7 128 R3 3 TMT02_R3 TMT02_R3 C_24 3 C_24_3
## 8 129 R4 3 TMT02_R4 TMT02_R4 C_48 3 C_48_3
## 17 126 R1 3 TMT05_R1 TMT05_R1 CEv3_0 3 CEv3_0_3
## 18 127 R2 3 TMT05_R2 TMT05_R2 CEv3_4 3 CEv3_4_3
## 19 128 R3 3 TMT05_R3 TMT05_R3 CEv3_24 3 CEv3_24_3
## 20 129 R4 3 TMT05_R4 TMT05_R4 CEv3_48 3 CEv3_48_3
## 25 126 R1 2 TMT07_R1 TMT07_R1 C_0 2 C_0_2
## 26 127 R2 2 TMT07_R2 TMT07_R2 C_4 2 C_4_2
## 27 128 R3 2 TMT07_R3 TMT07_R3 C_24 2 C_24_2
## 28 129 R4 2 TMT07_R4 TMT07_R4 C_48 2 C_48_2
## 37 126 R1 2 TMT10_R1 TMT10_R1 CEv3_0 2 CEv3_0_2
## 38 127 R2 2 TMT10_R2 TMT10_R2 CEv3_4 2 CEv3_4_2
## 39 128 R3 2 TMT10_R3 TMT10_R3 CEv3_24 2 CEv3_24_2
## 40 129 R4 2 TMT10_R4 TMT10_R4 CEv3_48 2 CEv3_48_2
## 45 126 R1 1 TMT12_R1 TMT12_R1 C_0 1 C_0_1
## 46 127 R2 1 TMT12_R2 TMT12_R2 C_4 1 C_4_1
## 47 128 R3 1 TMT12_R3 TMT12_R3 C_24 1 C_24_1
## 48 129 R4 1 TMT12_R4 TMT12_R4 C_48 1 C_48_1
## 57 126 R1 1 TMT15_R1 TMT15_R1 CEv3_0 1 CEv3_0_1
## 58 127 R2 1 TMT15_R2 TMT15_R2 CEv3_4 1 CEv3_4_1
## 59 128 R3 1 TMT15_R3 TMT15_R3 CEv3_24 1 CEv3_24_1
## 60 129 R4 1 TMT15_R4 TMT15_R4 CEv3_48 1 CEv3_48_1
## 61 126 R1 3 TMT16_R1 TMT16_R1 G12_0 3 G12_0_3
## 62 127 R2 3 TMT16_R2 TMT16_R2 G12_4 3 G12_4_3
## 63 128 R3 3 TMT16_R3 TMT16_R3 G12_24 3 G12_24_3
## 64 129 R4 3 TMT16_R4 TMT16_R4 G12_48 3 G12_48_3
## 65 126 R1 3 TMT17_R1 TMT17_R1 G8_0 3 G8_0_3
## 66 127 R2 3 TMT17_R2 TMT17_R2 G8_4 3 G8_4_3
## 67 128 R3 3 TMT17_R3 TMT17_R3 G8_24 3 G8_24_3
## 68 129 R4 3 TMT17_R4 TMT17_R4 G8_48 3 G8_48_3
## 69 126 R1 3 TMT18_R1 TMT18_R1 G5_0 3 G5_0_3
## 70 127 R2 3 TMT18_R2 TMT18_R2 G5_4 3 G5_4_3
## 71 128 R3 3 TMT18_R3 TMT18_R3 G5_24 3 G5_24_3
## 72 129 R4 3 TMT18_R4 TMT18_R4 G5_48 3 G5_48_3
## 73 126 R1 3 TMT19_R1 TMT19_R1 G1_0 3 G1_0_3
## 74 127 R2 3 TMT19_R2 TMT19_R2 G1_4 3 G1_4_3
## 75 128 R3 3 TMT19_R3 TMT19_R3 G1_24 3 G1_24_3
## 76 129 R4 3 TMT19_R4 TMT19_R4 G1_48 3 G1_48_3
## 77 126 R1 3 TMT20_R1 TMT20_R1 E5_0 3 E5_0_3
## 78 127 R2 3 TMT20_R2 TMT20_R2 E5_4 3 E5_4_3
## 79 128 R3 3 TMT20_R3 TMT20_R3 E5_24 3 E5_24_3
## 80 129 R4 3 TMT20_R4 TMT20_R4 E5_48 3 E5_48_3
## 81 126 R1 3 TMT21_R1 TMT21_R1 E4_0 3 E4_0_3
## 82 127 R2 3 TMT21_R2 TMT21_R2 E4_4 3 E4_4_3
## 83 128 R3 3 TMT21_R3 TMT21_R3 E4_24 3 E4_24_3
## 84 129 R4 3 TMT21_R4 TMT21_R4 E4_48 3 E4_48_3
## 97 126 R1 2 TMT25_R1 TMT25_R1 G12_0 2 G12_0_2
## 98 127 R2 2 TMT25_R2 TMT25_R2 G12_4 2 G12_4_2
## 99 128 R3 2 TMT25_R3 TMT25_R3 G12_24 2 G12_24_2
## 100 129 R4 2 TMT25_R4 TMT25_R4 G12_48 2 G12_48_2
## 101 126 R1 2 TMT26_R1 TMT26_R1 G8_0 2 G8_0_2
## 102 127 R2 2 TMT26_R2 TMT26_R2 G8_4 2 G8_4_2
## 103 128 R3 2 TMT26_R3 TMT26_R3 G8_24 2 G8_24_2
## 104 129 R4 2 TMT26_R4 TMT26_R4 G8_48 2 G8_48_2
## 105 126 R1 2 TMT27_R1 TMT27_R1 G5_0 2 G5_0_2
## 106 127 R2 2 TMT27_R2 TMT27_R2 G5_4 2 G5_4_2
## 107 128 R3 2 TMT27_R3 TMT27_R3 G5_24 2 G5_24_2
## 108 129 R4 2 TMT27_R4 TMT27_R4 G5_48 2 G5_48_2
## 109 126 R1 2 TMT28_R1 TMT28_R1 G1_0 2 G1_0_2
## 110 127 R2 2 TMT28_R2 TMT28_R2 G1_4 2 G1_4_2
## 111 128 R3 2 TMT28_R3 TMT28_R3 G1_24 2 G1_24_2
## 112 129 R4 2 TMT28_R4 TMT28_R4 G1_48 2 G1_48_2
## 113 126 R1 2 TMT29_R1 TMT29_R1 E5_0 2 E5_0_2
## 114 127 R2 2 TMT29_R2 TMT29_R2 E5_4 2 E5_4_2
## 115 128 R3 2 TMT29_R3 TMT29_R3 E5_24 2 E5_24_2
## 116 129 R4 2 TMT29_R4 TMT29_R4 E5_48 2 E5_48_2
## 117 126 R1 2 TMT30_R1 TMT30_R1 E4_0 2 E4_0_2
## 118 127 R2 2 TMT30_R2 TMT30_R2 E4_4 2 E4_4_2
## 119 128 R3 2 TMT30_R3 TMT30_R3 E4_24 2 E4_24_2
## 120 129 R4 2 TMT30_R4 TMT30_R4 E4_48 2 E4_48_2
## 133 126 R1 1 TMT34_R1 TMT34_R1 G12_0 1 G12_0_1
## 134 127 R2 1 TMT34_R2 TMT34_R2 G12_4 1 G12_4_1
## 135 128 R3 1 TMT34_R3 TMT34_R3 G12_24 1 G12_24_1
## 136 129 R4 1 TMT34_R4 TMT34_R4 G12_48 1 G12_48_1
## 137 126 R1 1 TMT35_R1 TMT35_R1 G8_0 1 G8_0_1
## 138 127 R2 1 TMT35_R2 TMT35_R2 G8_4 1 G8_4_1
## 139 128 R3 1 TMT35_R3 TMT35_R3 G8_24 1 G8_24_1
## 140 129 R4 1 TMT35_R4 TMT35_R4 G8_48 1 G8_48_1
## 141 126 R1 1 TMT36_R1 TMT36_R1 G5_0 1 G5_0_1
## 142 127 R2 1 TMT36_R2 TMT36_R2 G5_4 1 G5_4_1
## 143 128 R3 1 TMT36_R3 TMT36_R3 G5_24 1 G5_24_1
## 144 129 R4 1 TMT36_R4 TMT36_R4 G5_48 1 G5_48_1
## 145 126 R1 1 TMT37_R1 TMT37_R1 G1_0 1 G1_0_1
## 146 127 R2 1 TMT37_R2 TMT37_R2 G1_4 1 G1_4_1
## 147 128 R3 1 TMT37_R3 TMT37_R3 G1_24 1 G1_24_1
## 148 129 R4 1 TMT37_R4 TMT37_R4 G1_48 1 G1_48_1
## 149 126 R1 1 TMT38_R1 TMT38_R1 E5_0 1 E5_0_1
## 150 127 R2 1 TMT38_R2 TMT38_R2 E5_4 1 E5_4_1
## 151 128 R3 1 TMT38_R3 TMT38_R3 E5_24 1 E5_24_1
## 152 129 R4 1 TMT38_R4 TMT38_R4 E5_48 1 E5_48_1
## 153 126 R1 1 TMT39_R1 TMT39_R1 E4_0 1 E4_0_1
## 154 127 R2 1 TMT39_R2 TMT39_R2 E4_4 1 E4_4_1
## 155 128 R3 1 TMT39_R3 TMT39_R3 E4_24 1 E4_24_1
## 156 129 R4 1 TMT39_R4 TMT39_R4 E4_48 1 E4_48_1
## EGFR_TKI_sen measure
## 5 Sensitive Intensity
## 6 Sensitive Intensity
## 7 Sensitive Intensity
## 8 Sensitive Intensity
## 17 Sensitive Intensity
## 18 Sensitive Intensity
## 19 Sensitive Intensity
## 20 Sensitive Intensity
## 25 Sensitive Intensity
## 26 Sensitive Intensity
## 27 Sensitive Intensity
## 28 Sensitive Intensity
## 37 Sensitive Intensity
## 38 Sensitive Intensity
## 39 Sensitive Intensity
## 40 Sensitive Intensity
## 45 Sensitive Intensity
## 46 Sensitive Intensity
## 47 Sensitive Intensity
## 48 Sensitive Intensity
## 57 Sensitive Intensity
## 58 Sensitive Intensity
## 59 Sensitive Intensity
## 60 Sensitive Intensity
## 61 Resistant Intensity
## 62 Resistant Intensity
## 63 Resistant Intensity
## 64 Resistant Intensity
## 65 Resistant Intensity
## 66 Resistant Intensity
## 67 Resistant Intensity
## 68 Resistant Intensity
## 69 Resistant Intensity
## 70 Resistant Intensity
## 71 Resistant Intensity
## 72 Resistant Intensity
## 73 Resistant Intensity
## 74 Resistant Intensity
## 75 Resistant Intensity
## 76 Resistant Intensity
## 77 Resistant Intensity
## 78 Resistant Intensity
## 79 Resistant Intensity
## 80 Resistant Intensity
## 81 Resistant Intensity
## 82 Resistant Intensity
## 83 Resistant Intensity
## 84 Resistant Intensity
## 97 Resistant Intensity
## 98 Resistant Intensity
## 99 Resistant Intensity
## 100 Resistant Intensity
## 101 Resistant Intensity
## 102 Resistant Intensity
## 103 Resistant Intensity
## 104 Resistant Intensity
## 105 Resistant Intensity
## 106 Resistant Intensity
## 107 Resistant Intensity
## 108 Resistant Intensity
## 109 Resistant Intensity
## 110 Resistant Intensity
## 111 Resistant Intensity
## 112 Resistant Intensity
## 113 Resistant Intensity
## 114 Resistant Intensity
## 115 Resistant Intensity
## 116 Resistant Intensity
## 117 Resistant Intensity
## 118 Resistant Intensity
## 119 Resistant Intensity
## 120 Resistant Intensity
## 133 Resistant Intensity
## 134 Resistant Intensity
## 135 Resistant Intensity
## 136 Resistant Intensity
## 137 Resistant Intensity
## 138 Resistant Intensity
## 139 Resistant Intensity
## 140 Resistant Intensity
## 141 Resistant Intensity
## 142 Resistant Intensity
## 143 Resistant Intensity
## 144 Resistant Intensity
## 145 Resistant Intensity
## 146 Resistant Intensity
## 147 Resistant Intensity
## 148 Resistant Intensity
## 149 Resistant Intensity
## 150 Resistant Intensity
## 151 Resistant Intensity
## 152 Resistant Intensity
## 153 Resistant Intensity
## 154 Resistant Intensity
## 155 Resistant Intensity
## 156 Resistant Intensity
TMT_samples_Par <- subset(TMT_samples_all,TMT_samples_all$Cell.Line %in% c("C","CEv3"))
# Subset DEP object
DEP_TMT_Par <- se_mibs_TMT_bc[,paste0(TMT_samples_Par$name)]
# Save sample sheet
write.csv(TMT_samples_Par, file=paste0(output_dir,"DEP_TMT_Par",".csv"))
# Save DEP_2019_parental as RDS
saveRDS(DEP_TMT_Par, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","DEP_TMT_Par",".rds"))
# PCA with all genes
# Create pca object
pcaData <- PCAtools::pca(assay(DEP_TMT_Par), metadata = colData(DEP_TMT_Par))
# Save pca data
write.csv(pcaData$rotated, file =paste0(output_dir,"pcaData",".csv"))
# Set colors
color_vector <-
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Cell.Line',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
pairsplot
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_","pairsplot", ".pdf"),
plot = pairsplot
)
## Saving 7 x 5 in image
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Cell.Line",
colkey= color_vector,
shape = "Timepoint",
legendPosition = 'right' )
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
p1
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_vsd_500_","cell_line",".pdf"),
plot = p1
)
## Saving 7 x 5 in image
# calculate sample euclidian distances
sampleDists <- dist(t(assay(DEP_TMT_Par)))
# plot sample euclidian distances via a heatmap
sampleDistMatrix <- as.matrix( sampleDists )
# Complex Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_TMT_Par@colData$Cell.Line,
Time = DEP_TMT_Par@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
))
)
# Heatmap of euclidian distances
ComplexHeatmap::Heatmap(
sampleDistMatrix,
name = " ",
column_title = "Eculidian Distances",
show_column_names = FALSE,
show_row_names = FALSE,
clustering_distance_rows = "euclidean",
clustering_distance_columns = "euclidean",
clustering_method_rows = "complete",
clustering_method_columns = "complete",
col = colorRampPalette( rev(brewer.pal(9, "Blues")) )(255),
top_annotation = annotation_col
)
# DEP_TMT_Par
# Directories
dir.create(paste0(output_dir,"Heatmap_TVG"))
output_dir_Heatmap_TVG <- paste0(output_dir,"Heatmap_TVG/")
dir.create(paste0(graph_dir,"Heatmap_TVG"))
graph_dir_Heatmap_TVG <- paste0(graph_dir,"Heatmap_TVG/")
# pHeatmap of Top X DEG on transformed data
tvg <- c(100,50,25,10)
topVarGenesX_list <- list()
# pHeatmap of Top X DEG on transformed data
# Extract out distances
mat_list <- list()
for (i in seq_along(tvg)) {
topVarGenesX_list[[paste0(tvg[i])]] <- head( order( rowVars( as.matrix(assay(DEP_TMT_Par)) ), decreasing=TRUE ), tvg[[i]] )
}
for (i in seq_along(tvg)) {
mat_list[[paste0(tvg[i])]] <- assay(DEP_TMT_Par)[ topVarGenesX_list[[i]], ]
}
# Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_TMT_Par@colData$Cell.Line,
Time = DEP_TMT_Par@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
))
)
# Generate Unlabeled Heatmap
p_list <- list()
for (i in seq_along(mat_list)) {
p_list[[paste0(names(mat_list)[[i]])]] <- ComplexHeatmap::Heatmap(
t(scale(t(mat_list[[i]]))),
top_annotation = annotation_col,
name = " ",
column_title = paste0(names(mat_list)[i],"_TVG"),
show_column_names = F,
show_row_names = F
)
print(p_list[[i]])
}
# Save Heatmap
for (i in seq_along(p_list)) {
pdf(file=paste0(graph_dir_Heatmap_TVG, "CHeatmap_" , names(p_list)[[i]], ".pdf"))
draw(p_list[[i]])
dev.off()
}
# Generate labeled Heatmap
p_list <- list()
for (i in seq_along(mat_list)) {
p_list[[paste0(names(mat_list)[[i]])]] <- ComplexHeatmap::Heatmap(
t(scale(t(mat_list[[i]]))),
#column_order=row.names(DEP_TMT_Par@colData[order(DEP_TMT_Par@colData$clid),]),
top_annotation = annotation_col,
column_split = factor(DEP_TMT_Par@colData$clid),
name = " ",
column_title = paste0(names(mat_list)[i],"_TVG"),
show_column_names = F,
show_row_names = T
)
print(p_list[[i]])
}
# Save Heatmap
for (i in seq_along(p_list)) {
pdf(file=paste0(graph_dir_Heatmap_TVG, "CHeatmapLabel_" , names(p_list)[[i]], ".pdf"))
draw(p_list[[i]])
dev.off()
}
# Graphics termination
if(!is.null(dev.list())) dev.off()
## null device
## 1
if(!is.null(dev.list())) graphics.off()
# Directories
dir.create(paste0(output_dir,"Limma_res"))
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# comps
comps_factor <- as.vector(factor(paste0(colData(DEP_TMT_Par)$condition)))
comps_df <- crossing(comp_num=comps_factor, comp_denom=comps_factor)
comps_df <- subset(comps_df, as.character(comps_df$comp_num) != as.character(comps_df$comp_denom))
# All comps
comps_df_all <- comps_df
comps_df_all <- cbind(comp_type="condition",comps_df_all)
comps_df_all <- cbind(comp_no=1:nrow(comps_df_all),comps_df_all)
comps_df_all$comp_name <- paste(comps_df_all$comp_num, "vs", comps_df_all$comp_denom, sep = "-")
comps_df_all$comp <- paste(comps_df_all$comp_num,comps_df_all$comp_denom, sep="-")
write.csv(comps_df_all, file=paste0(output_dir_res,"comps_all.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
comps_all_subset <- comps_df_all[,2:4]
# Unique comps only (for only unique combo)
comps_df_unique <- comps_df[!duplicated(t(apply(comps_df, 1, sort))), ]
comps_df_unique <- cbind(comp_type="condition",comps_df_unique )
comps_df_unique <- cbind(comp_no=1:nrow(comps_df_unique),comps_df_unique)
comps_df_unique$comp_name <- paste(comps_df_unique$comp_num, "vs", comps_df_unique$comp_denom, sep = "-")
comps_df_unique$comp <- paste(comps_df_unique$comp_num,comps_df_unique$comp_denom, sep="-")
write.csv(comps_df_unique, file=paste0(output_dir_res,"comps_unique.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
comps_unique_subset <- comps_df_unique[,2:4]
# Comp list to use (all comps)
comps <- comps_df_all
# Extract
# Results
# mibs_comp_list
# Directories
dir.create(paste0(output_dir,"Limma_res"))
## Warning in dir.create(paste0(output_dir, "Limma_res")):
## '/Data/Output/MIB-DYN/DEP_TMT_Par/Limma_res' already exists
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
## Warning in dir.create(paste0(graph_dir, "Limma_res")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_Par/Limma_res' already exists
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# Extract results
DEP_TMT_limmaRes <- list()
for (i in seq_along(comps$comp)){
DEP_TMT_limmaRes[[comps$comp[i]]] <-
mibs_comp_list[[paste0(comps$comp[i])]]
}
# save results
for (i in seq_along(DEP_TMT_limmaRes)) {
write.csv(
DEP_TMT_limmaRes[[i]],
file = paste0(output_dir_res,"comp_", i, "_", names(DEP_TMT_limmaRes)[i], ".csv"),
row.names = TRUE
)
}
# Directories
dir.create(paste0(output_dir,"Limma_res"))
## Warning in dir.create(paste0(output_dir, "Limma_res")):
## '/Data/Output/MIB-DYN/DEP_TMT_Par/Limma_res' already exists
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
## Warning in dir.create(paste0(graph_dir, "Limma_res")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_Par/Limma_res' already exists
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# Extract comps of interest
coi <- comps_df_all[comps_df_all$comp_denom=="C_0",]
comp_list_oi <- DEP_TMT_limmaRes[names(DEP_TMT_limmaRes) %in% coi$comp]
comp_list_oi <- lapply(comp_list_oi,data.frame)
# Label list of DF
for (i in seq_along(comp_list_oi)){
colnames(comp_list_oi[[i]]) <- paste0(colnames(comp_list_oi[[i]]),"_",names(comp_list_oi)[[i]])
comp_list_oi[[i]]$Gene_ID <- row.names(comp_list_oi[[i]])
}
# Left_join comps of interest to 1x spreadsheet
comp_list_oi_master <- Reduce(function(x, y) left_join(x, y, by="Gene_ID"), comp_list_oi)
row.names(comp_list_oi_master) <- comp_list_oi_master$Gene_ID
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)=="Gene_ID")]
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)%in% paste0(grep("gene_",colnames(comp_list_oi_master), value = T)))]
# Subset to spreadsheets
comp_list_oi_padj <- comp_list_oi_master[,grep("adj.P.Val_", colnames(comp_list_oi_master))]
comp_list_oi_pvalue <- comp_list_oi_master[,grep("P.Value_", colnames(comp_list_oi_master))]
comp_list_oi_l2fc <- comp_list_oi_master[,grep("logFC_", colnames(comp_list_oi_master))]
# Save spreadsheets
write.csv(comp_list_oi_padj, file = paste0(output_dir_res,"All_coi_","padj",".csv"))
write.csv(comp_list_oi_pvalue, file = paste0(output_dir_res,"All_coi_","pvalue",".csv"))
write.csv(comp_list_oi_l2fc, file = paste0(output_dir_res,"All_coi_","l2fc",".csv"))
# Directory for volcano plots
dir.create(paste0(graph_dir,"/","volcano"))
graph_dir_vol <- paste0(graph_dir,"volcano/")
# Plot Volcano
volcano_plots_ls <- list()
for (i in seq_along(DEP_TMT_limmaRes)){
plot(volcano_plots_ls[[names(DEP_TMT_limmaRes)[i]]] <-
EnhancedVolcano(
DEP_TMT_limmaRes[[i]],
lab=row.names(DEP_TMT_limmaRes[[i]]),
x="logFC",
y="adj.P.Val",
title= paste0("MIBs ",names(DEP_TMT_limmaRes)[[i]]),
pCutoff =qval,
FCcutoff =lfc,
pointSize = 2.0,
labSize = 3.0
))}
# Save Volcano
for (i in seq_along(volcano_plots_ls)){
ggsave(filename = paste0(graph_dir_vol,"comp_", i, "_", names(volcano_plots_ls)[[i]],".pdf"), plot=volcano_plots_ls[[i]])
}
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
# Graphics termination
if(!is.null(dev.list())) dev.off()
## null device
## 1
if(!is.null(dev.list())) graphics.off()
# Directories for DEP_TMT_BL_Res
dir.create(paste0(MIB_graphs,"DEP_TMT_BL_Res"))
dir.create(paste0(MIB_output,"DEP_TMT_BL_Res"))
graph_dir <- paste0(MIB_graphs,"DEP_TMT_BL_Res/")
output_dir <- paste0(MIB_output,"DEP_TMT_BL_Res/")
# Subset
TMT_samples_all
## Prep Run Number Sample Cell.Line clid Timepoint serum Harvest
## 5 Prep_01 TMT02 6 P0298 C 701 0 starve 180807
## 6 Prep_01 TMT02 7 P0299 C 701 4 starve 180807
## 7 Prep_01 TMT02 8 P0300 C 701 24 starve 180808
## 8 Prep_01 TMT02 9 P0301 C 701 48 starve 180809
## 17 Prep_02 TMT05 21 P0294 CEv3 692 0 starve 180807
## 18 Prep_03 TMT05 22 P0295 CEv3 692 4 starve 180807
## 19 Prep_03 TMT05 23 P0296 CEv3 692 24 starve 180808
## 20 Prep_02 TMT05 24 P0297 CEv3 692 48 starve 180809
## 25 Prep_02 TMT07 31 P0117 C 701 0 starve 180705
## 26 Prep_02 TMT07 32 P0118 C 701 4 starve 180705
## 27 Prep_02 TMT07 33 P0119 C 701 24 starve 180706
## 28 Prep_02 TMT07 34 P0120 C 701 48 starve 180708
## 37 Prep_03 TMT10 46 P0290 CEv3 692 0 starve 180806
## 38 Prep_03 TMT10 47 P0291 CEv3 692 4 starve 180806
## 39 Prep_03 TMT10 48 P0292 CEv3 692 24 starve 180807
## 40 Prep_03 TMT10 49 P0293 CEv3 692 48 starve 180808
## 45 Prep_04 TMT12 56 P0113 C 701 0 starve 180704
## 46 Prep_04 TMT12 57 P0114 C 701 4 starve 180704
## 47 Prep_04 TMT12 58 P0115 C 701 24 starve 180705
## 48 Prep_04 TMT12 59 P0116 C 701 48 starve 180706
## 57 Prep_04 TMT15 71 P0286 CEv3 692 0 starve 180805
## 58 Prep_04 TMT15 72 P0287 CEv3 692 4 starve 180805
## 59 Prep_04 TMT15 73 P0288 CEv3 692 24 starve 180806
## 60 Prep_04 TMT15 74 P0289 CEv3 692 48 starve 180807
## 61 Prep_05 TMT16 76 P0368 G12 698 0 starve 180904
## 62 Prep_05 TMT16 77 P0369 G12 698 4 starve 180904
## 63 Prep_05 TMT16 78 P0370 G12 698 24 starve 180905
## 64 Prep_05 TMT16 79 P0371 G12 698 48 starve 180906
## 65 Prep_05 TMT17 81 P0356 G8 697 0 starve 180826
## 66 Prep_05 TMT17 82 P0357 G8 697 4 starve 180826
## 67 Prep_05 TMT17 83 P0358 G8 697 24 starve 180827
## 68 Prep_05 TMT17 84 P0359 G8 697 48 starve 180828
## 69 Prep_05 TMT18 86 P0344 G5 696 0 starve 180905
## 70 Prep_05 TMT18 87 P0345 G5 696 4 starve 180905
## 71 Prep_05 TMT18 88 P0346 G5 696 24 starve 180906
## 72 Prep_05 TMT18 89 P0347 G5 696 48 starve 180907
## 73 Prep_05 TMT19 91 P0332 G1 695 0 starve 180826
## 74 Prep_05 TMT19 92 P0333 G1 695 4 starve 180826
## 75 Prep_05 TMT19 93 P0334 G1 695 24 starve 180827
## 76 Prep_05 TMT19 94 P0335 G1 695 48 starve 180828
## 77 Prep_06 TMT20 96 P0204 E5 694 0 starve 180724
## 78 Prep_06 TMT20 97 P0205 E5 694 4 starve 180724
## 79 Prep_06 TMT20 98 P0206 E5 694 24 starve 180725
## 80 Prep_06 TMT20 99 P0207 E5 694 48 starve 180726
## 81 Prep_06 TMT21 101 P0189 E4 693 0 starve 180724
## 82 Prep_06 TMT21 102 P0190 E4 693 4 starve 180724
## 83 Prep_06 TMT21 103 P0191 E4 693 24 starve 180724
## 84 Prep_06 TMT21 104 P0192 E4 693 48 starve 180725
## 97 Prep_07 TMT25 121 P0364 G12 698 0 starve 180905
## 98 Prep_07 TMT25 122 P0365 G12 698 4 starve 180905
## 99 Prep_07 TMT25 123 P0366 G12 698 24 starve 180906
## 100 Prep_07 TMT25 124 P0367 G12 698 48 starve 180907
## 101 Prep_07 TMT26 126 P0352 G8 697 0 starve 180825
## 102 Prep_07 TMT26 127 P0353 G8 697 4 starve 180825
## 103 Prep_07 TMT26 128 P0354 G8 697 24 starve 180826
## 104 Prep_07 TMT26 129 P0355 G8 697 48 starve 180827
## 105 Prep_07 TMT27 131 P0340 G5 696 0 starve 180906
## 106 Prep_07 TMT27 132 P0341 G5 696 4 starve 180906
## 107 Prep_07 TMT27 133 P0342 G5 696 24 starve 180907
## 108 Prep_07 TMT27 134 P0343 G5 696 48 starve 180908
## 109 Prep_08 TMT28 136 P0328 G1 695 0 starve 180825
## 110 Prep_08 TMT28 137 P0329 G1 695 4 starve 180825
## 111 Prep_08 TMT28 138 P0330 G1 695 24 starve 180826
## 112 Prep_08 TMT28 139 P0331 G1 695 48 starve 180827
## 113 Prep_08 TMT29 141 P0200 E5 694 0 starve 180723
## 114 Prep_08 TMT29 142 P0201 E5 694 4 starve 180723
## 115 Prep_08 TMT29 143 P0202 E5 694 24 starve 180724
## 116 Prep_08 TMT29 144 P0203 E5 694 48 starve 180725
## 117 Prep_08 TMT30 146 P0185 E4 693 0 starve 180723
## 118 Prep_08 TMT30 147 P0186 E4 693 4 starve 180723
## 119 Prep_08 TMT30 148 P0187 E4 693 24 starve 180724
## 120 Prep_08 TMT30 149 P0188 E4 693 48 starve 180725
## 133 Prep_09 TMT34 166 P0360 G12 698 0 starve 180826
## 134 Prep_09 TMT34 167 P0361 G12 698 4 starve 180826
## 135 Prep_09 TMT34 168 P0362 G12 698 24 starve 180827
## 136 Prep_09 TMT34 169 P0363 G12 698 48 starve 180828
## 137 Prep_10 TMT35 171 P0348 G8 697 0 starve 180824
## 138 Prep_10 TMT35 172 P0349 G8 697 4 starve 180824
## 139 Prep_10 TMT35 173 P0350 G8 697 24 starve 180825
## 140 Prep_10 TMT35 174 P0351 G8 697 48 starve 180826
## 141 Prep_10 TMT36 176 P0336 G5 696 0 starve 180826
## 142 Prep_10 TMT36 177 P0337 G5 696 4 starve 180826
## 143 Prep_10 TMT36 178 P0338 G5 696 24 starve 180827
## 144 Prep_10 TMT36 179 P0339 G5 696 48 starve 180828
## 145 Prep_10 TMT37 181 P0324 G1 695 0 starve 180824
## 146 Prep_10 TMT37 182 P0325 G1 695 4 starve 180824
## 147 Prep_10 TMT37 183 P0326 G1 695 24 starve 180825
## 148 Prep_10 TMT37 184 P0327 G1 695 48 starve 180826
## 149 Prep_10 TMT38 186 P0196 E5 694 0 starve 180722
## 150 Prep_10 TMT38 187 P0197 E5 694 4 starve 180722
## 151 Prep_10 TMT38 188 P0198 E5 694 24 starve 180723
## 152 Prep_10 TMT38 189 P0199 E5 694 48 starve 180724
## 153 Prep_11 TMT39 191 P0181 E4 693 0 starve 180722
## 154 Prep_11 TMT39 192 P0182 E4 693 4 starve 180722
## 155 Prep_11 TMT39 193 P0183 E4 693 24 starve 180723
## 156 Prep_11 TMT39 194 P0184 E4 693 48 starve 180724
## Label_num Tag Exp_rep TMT.Reporter label condition replicate name
## 5 126 R1 3 TMT02_R1 TMT02_R1 C_0 3 C_0_3
## 6 127 R2 3 TMT02_R2 TMT02_R2 C_4 3 C_4_3
## 7 128 R3 3 TMT02_R3 TMT02_R3 C_24 3 C_24_3
## 8 129 R4 3 TMT02_R4 TMT02_R4 C_48 3 C_48_3
## 17 126 R1 3 TMT05_R1 TMT05_R1 CEv3_0 3 CEv3_0_3
## 18 127 R2 3 TMT05_R2 TMT05_R2 CEv3_4 3 CEv3_4_3
## 19 128 R3 3 TMT05_R3 TMT05_R3 CEv3_24 3 CEv3_24_3
## 20 129 R4 3 TMT05_R4 TMT05_R4 CEv3_48 3 CEv3_48_3
## 25 126 R1 2 TMT07_R1 TMT07_R1 C_0 2 C_0_2
## 26 127 R2 2 TMT07_R2 TMT07_R2 C_4 2 C_4_2
## 27 128 R3 2 TMT07_R3 TMT07_R3 C_24 2 C_24_2
## 28 129 R4 2 TMT07_R4 TMT07_R4 C_48 2 C_48_2
## 37 126 R1 2 TMT10_R1 TMT10_R1 CEv3_0 2 CEv3_0_2
## 38 127 R2 2 TMT10_R2 TMT10_R2 CEv3_4 2 CEv3_4_2
## 39 128 R3 2 TMT10_R3 TMT10_R3 CEv3_24 2 CEv3_24_2
## 40 129 R4 2 TMT10_R4 TMT10_R4 CEv3_48 2 CEv3_48_2
## 45 126 R1 1 TMT12_R1 TMT12_R1 C_0 1 C_0_1
## 46 127 R2 1 TMT12_R2 TMT12_R2 C_4 1 C_4_1
## 47 128 R3 1 TMT12_R3 TMT12_R3 C_24 1 C_24_1
## 48 129 R4 1 TMT12_R4 TMT12_R4 C_48 1 C_48_1
## 57 126 R1 1 TMT15_R1 TMT15_R1 CEv3_0 1 CEv3_0_1
## 58 127 R2 1 TMT15_R2 TMT15_R2 CEv3_4 1 CEv3_4_1
## 59 128 R3 1 TMT15_R3 TMT15_R3 CEv3_24 1 CEv3_24_1
## 60 129 R4 1 TMT15_R4 TMT15_R4 CEv3_48 1 CEv3_48_1
## 61 126 R1 3 TMT16_R1 TMT16_R1 G12_0 3 G12_0_3
## 62 127 R2 3 TMT16_R2 TMT16_R2 G12_4 3 G12_4_3
## 63 128 R3 3 TMT16_R3 TMT16_R3 G12_24 3 G12_24_3
## 64 129 R4 3 TMT16_R4 TMT16_R4 G12_48 3 G12_48_3
## 65 126 R1 3 TMT17_R1 TMT17_R1 G8_0 3 G8_0_3
## 66 127 R2 3 TMT17_R2 TMT17_R2 G8_4 3 G8_4_3
## 67 128 R3 3 TMT17_R3 TMT17_R3 G8_24 3 G8_24_3
## 68 129 R4 3 TMT17_R4 TMT17_R4 G8_48 3 G8_48_3
## 69 126 R1 3 TMT18_R1 TMT18_R1 G5_0 3 G5_0_3
## 70 127 R2 3 TMT18_R2 TMT18_R2 G5_4 3 G5_4_3
## 71 128 R3 3 TMT18_R3 TMT18_R3 G5_24 3 G5_24_3
## 72 129 R4 3 TMT18_R4 TMT18_R4 G5_48 3 G5_48_3
## 73 126 R1 3 TMT19_R1 TMT19_R1 G1_0 3 G1_0_3
## 74 127 R2 3 TMT19_R2 TMT19_R2 G1_4 3 G1_4_3
## 75 128 R3 3 TMT19_R3 TMT19_R3 G1_24 3 G1_24_3
## 76 129 R4 3 TMT19_R4 TMT19_R4 G1_48 3 G1_48_3
## 77 126 R1 3 TMT20_R1 TMT20_R1 E5_0 3 E5_0_3
## 78 127 R2 3 TMT20_R2 TMT20_R2 E5_4 3 E5_4_3
## 79 128 R3 3 TMT20_R3 TMT20_R3 E5_24 3 E5_24_3
## 80 129 R4 3 TMT20_R4 TMT20_R4 E5_48 3 E5_48_3
## 81 126 R1 3 TMT21_R1 TMT21_R1 E4_0 3 E4_0_3
## 82 127 R2 3 TMT21_R2 TMT21_R2 E4_4 3 E4_4_3
## 83 128 R3 3 TMT21_R3 TMT21_R3 E4_24 3 E4_24_3
## 84 129 R4 3 TMT21_R4 TMT21_R4 E4_48 3 E4_48_3
## 97 126 R1 2 TMT25_R1 TMT25_R1 G12_0 2 G12_0_2
## 98 127 R2 2 TMT25_R2 TMT25_R2 G12_4 2 G12_4_2
## 99 128 R3 2 TMT25_R3 TMT25_R3 G12_24 2 G12_24_2
## 100 129 R4 2 TMT25_R4 TMT25_R4 G12_48 2 G12_48_2
## 101 126 R1 2 TMT26_R1 TMT26_R1 G8_0 2 G8_0_2
## 102 127 R2 2 TMT26_R2 TMT26_R2 G8_4 2 G8_4_2
## 103 128 R3 2 TMT26_R3 TMT26_R3 G8_24 2 G8_24_2
## 104 129 R4 2 TMT26_R4 TMT26_R4 G8_48 2 G8_48_2
## 105 126 R1 2 TMT27_R1 TMT27_R1 G5_0 2 G5_0_2
## 106 127 R2 2 TMT27_R2 TMT27_R2 G5_4 2 G5_4_2
## 107 128 R3 2 TMT27_R3 TMT27_R3 G5_24 2 G5_24_2
## 108 129 R4 2 TMT27_R4 TMT27_R4 G5_48 2 G5_48_2
## 109 126 R1 2 TMT28_R1 TMT28_R1 G1_0 2 G1_0_2
## 110 127 R2 2 TMT28_R2 TMT28_R2 G1_4 2 G1_4_2
## 111 128 R3 2 TMT28_R3 TMT28_R3 G1_24 2 G1_24_2
## 112 129 R4 2 TMT28_R4 TMT28_R4 G1_48 2 G1_48_2
## 113 126 R1 2 TMT29_R1 TMT29_R1 E5_0 2 E5_0_2
## 114 127 R2 2 TMT29_R2 TMT29_R2 E5_4 2 E5_4_2
## 115 128 R3 2 TMT29_R3 TMT29_R3 E5_24 2 E5_24_2
## 116 129 R4 2 TMT29_R4 TMT29_R4 E5_48 2 E5_48_2
## 117 126 R1 2 TMT30_R1 TMT30_R1 E4_0 2 E4_0_2
## 118 127 R2 2 TMT30_R2 TMT30_R2 E4_4 2 E4_4_2
## 119 128 R3 2 TMT30_R3 TMT30_R3 E4_24 2 E4_24_2
## 120 129 R4 2 TMT30_R4 TMT30_R4 E4_48 2 E4_48_2
## 133 126 R1 1 TMT34_R1 TMT34_R1 G12_0 1 G12_0_1
## 134 127 R2 1 TMT34_R2 TMT34_R2 G12_4 1 G12_4_1
## 135 128 R3 1 TMT34_R3 TMT34_R3 G12_24 1 G12_24_1
## 136 129 R4 1 TMT34_R4 TMT34_R4 G12_48 1 G12_48_1
## 137 126 R1 1 TMT35_R1 TMT35_R1 G8_0 1 G8_0_1
## 138 127 R2 1 TMT35_R2 TMT35_R2 G8_4 1 G8_4_1
## 139 128 R3 1 TMT35_R3 TMT35_R3 G8_24 1 G8_24_1
## 140 129 R4 1 TMT35_R4 TMT35_R4 G8_48 1 G8_48_1
## 141 126 R1 1 TMT36_R1 TMT36_R1 G5_0 1 G5_0_1
## 142 127 R2 1 TMT36_R2 TMT36_R2 G5_4 1 G5_4_1
## 143 128 R3 1 TMT36_R3 TMT36_R3 G5_24 1 G5_24_1
## 144 129 R4 1 TMT36_R4 TMT36_R4 G5_48 1 G5_48_1
## 145 126 R1 1 TMT37_R1 TMT37_R1 G1_0 1 G1_0_1
## 146 127 R2 1 TMT37_R2 TMT37_R2 G1_4 1 G1_4_1
## 147 128 R3 1 TMT37_R3 TMT37_R3 G1_24 1 G1_24_1
## 148 129 R4 1 TMT37_R4 TMT37_R4 G1_48 1 G1_48_1
## 149 126 R1 1 TMT38_R1 TMT38_R1 E5_0 1 E5_0_1
## 150 127 R2 1 TMT38_R2 TMT38_R2 E5_4 1 E5_4_1
## 151 128 R3 1 TMT38_R3 TMT38_R3 E5_24 1 E5_24_1
## 152 129 R4 1 TMT38_R4 TMT38_R4 E5_48 1 E5_48_1
## 153 126 R1 1 TMT39_R1 TMT39_R1 E4_0 1 E4_0_1
## 154 127 R2 1 TMT39_R2 TMT39_R2 E4_4 1 E4_4_1
## 155 128 R3 1 TMT39_R3 TMT39_R3 E4_24 1 E4_24_1
## 156 129 R4 1 TMT39_R4 TMT39_R4 E4_48 1 E4_48_1
## EGFR_TKI_sen measure
## 5 Sensitive Intensity
## 6 Sensitive Intensity
## 7 Sensitive Intensity
## 8 Sensitive Intensity
## 17 Sensitive Intensity
## 18 Sensitive Intensity
## 19 Sensitive Intensity
## 20 Sensitive Intensity
## 25 Sensitive Intensity
## 26 Sensitive Intensity
## 27 Sensitive Intensity
## 28 Sensitive Intensity
## 37 Sensitive Intensity
## 38 Sensitive Intensity
## 39 Sensitive Intensity
## 40 Sensitive Intensity
## 45 Sensitive Intensity
## 46 Sensitive Intensity
## 47 Sensitive Intensity
## 48 Sensitive Intensity
## 57 Sensitive Intensity
## 58 Sensitive Intensity
## 59 Sensitive Intensity
## 60 Sensitive Intensity
## 61 Resistant Intensity
## 62 Resistant Intensity
## 63 Resistant Intensity
## 64 Resistant Intensity
## 65 Resistant Intensity
## 66 Resistant Intensity
## 67 Resistant Intensity
## 68 Resistant Intensity
## 69 Resistant Intensity
## 70 Resistant Intensity
## 71 Resistant Intensity
## 72 Resistant Intensity
## 73 Resistant Intensity
## 74 Resistant Intensity
## 75 Resistant Intensity
## 76 Resistant Intensity
## 77 Resistant Intensity
## 78 Resistant Intensity
## 79 Resistant Intensity
## 80 Resistant Intensity
## 81 Resistant Intensity
## 82 Resistant Intensity
## 83 Resistant Intensity
## 84 Resistant Intensity
## 97 Resistant Intensity
## 98 Resistant Intensity
## 99 Resistant Intensity
## 100 Resistant Intensity
## 101 Resistant Intensity
## 102 Resistant Intensity
## 103 Resistant Intensity
## 104 Resistant Intensity
## 105 Resistant Intensity
## 106 Resistant Intensity
## 107 Resistant Intensity
## 108 Resistant Intensity
## 109 Resistant Intensity
## 110 Resistant Intensity
## 111 Resistant Intensity
## 112 Resistant Intensity
## 113 Resistant Intensity
## 114 Resistant Intensity
## 115 Resistant Intensity
## 116 Resistant Intensity
## 117 Resistant Intensity
## 118 Resistant Intensity
## 119 Resistant Intensity
## 120 Resistant Intensity
## 133 Resistant Intensity
## 134 Resistant Intensity
## 135 Resistant Intensity
## 136 Resistant Intensity
## 137 Resistant Intensity
## 138 Resistant Intensity
## 139 Resistant Intensity
## 140 Resistant Intensity
## 141 Resistant Intensity
## 142 Resistant Intensity
## 143 Resistant Intensity
## 144 Resistant Intensity
## 145 Resistant Intensity
## 146 Resistant Intensity
## 147 Resistant Intensity
## 148 Resistant Intensity
## 149 Resistant Intensity
## 150 Resistant Intensity
## 151 Resistant Intensity
## 152 Resistant Intensity
## 153 Resistant Intensity
## 154 Resistant Intensity
## 155 Resistant Intensity
## 156 Resistant Intensity
TMT_samples_BL_Res <- subset(TMT_samples_all,TMT_samples_all$Cell.Line %in% c("CEv3","E4","E5","G1","G5","G8","G12") & TMT_samples_all$Timepoint=="0" )
# Subset DEP object
DEP_TMT_BL_Res <- se_mibs_TMT_bc[,paste0(TMT_samples_BL_Res$name)]
# Save sample sheet
write.csv(TMT_samples_BL_Res, file=paste0(output_dir,"DEP_TMT_BL_Res",".csv"))
# Save DEP_2019_parental as RDS
saveRDS(DEP_TMT_BL_Res, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","DEP_TMT_BL_Res",".rds"))
# PCA with all genes
# Create pca object
pcaData <- PCAtools::pca(assay(DEP_TMT_BL_Res), metadata = colData(DEP_TMT_BL_Res))
# Save pca data
write.csv(pcaData$rotated, file =paste0(output_dir,"pcaData",".csv"))
# Set colors
color_vector <-
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Cell.Line',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
pairsplot
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_","pairsplot", ".pdf"),
plot = pairsplot
)
## Saving 7 x 5 in image
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Cell.Line",
colkey= color_vector,
shape = "Timepoint",
legendPosition = 'right' )
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
p1
# save graph as PDF
ggsave(
filename = paste0(graph_dir,"pca_vsd_500_","cell_line",".pdf"),
plot = p1
)
## Saving 7 x 5 in image
# calculate sample euclidian distances
sampleDists <- dist(t(assay(DEP_TMT_BL_Res)))
# plot sample euclidian distances via a heatmap
sampleDistMatrix <- as.matrix( sampleDists )
# Complex Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_TMT_BL_Res@colData$Cell.Line,
Time = DEP_TMT_BL_Res@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
))
)
# Heatmap of euclidian distances
ComplexHeatmap::Heatmap(
sampleDistMatrix,
name = " ",
column_title = "Eculidian Distances",
show_column_names = FALSE,
show_row_names = FALSE,
clustering_distance_rows = "euclidean",
clustering_distance_columns = "euclidean",
clustering_method_rows = "complete",
clustering_method_columns = "complete",
col = colorRampPalette( rev(brewer.pal(9, "Blues")) )(255),
top_annotation = annotation_col
)
# DEP_TMT_BL_Res
# Directories
dir.create(paste0(output_dir,"Heatmap_TVG"))
output_dir_Heatmap_TVG <- paste0(output_dir,"Heatmap_TVG/")
dir.create(paste0(graph_dir,"Heatmap_TVG"))
graph_dir_Heatmap_TVG <- paste0(graph_dir,"Heatmap_TVG/")
# pHeatmap of Top X DEG on transformed data
tvg <- c(100,50,25,10)
topVarGenesX_list <- list()
# pHeatmap of Top X DEG on transformed data
# Extract out distances
mat_list <- list()
for (i in seq_along(tvg)) {
topVarGenesX_list[[paste0(tvg[i])]] <- head( order( rowVars( as.matrix(assay(DEP_TMT_BL_Res)) ), decreasing=TRUE ), tvg[[i]] )
}
for (i in seq_along(tvg)) {
mat_list[[paste0(tvg[i])]] <- assay(DEP_TMT_BL_Res)[ topVarGenesX_list[[i]], ]
}
# Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_TMT_BL_Res@colData$Cell.Line,
Time = DEP_TMT_BL_Res@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
))
)
# Generate Unlabeled Heatmap
p_list <- list()
for (i in seq_along(mat_list)) {
p_list[[paste0(names(mat_list)[[i]])]] <- ComplexHeatmap::Heatmap(
t(scale(t(mat_list[[i]]))),
top_annotation = annotation_col,
name = " ",
column_title = paste0(names(mat_list)[i],"_TVG"),
show_column_names = F,
show_row_names = F
)
print(p_list[[i]])
}
# Save Heatmap
for (i in seq_along(p_list)) {
pdf(file=paste0(graph_dir_Heatmap_TVG, "CHeatmap_" , names(p_list)[[i]], ".pdf"))
draw(p_list[[i]])
dev.off()
}
# Generate labeled Heatmap
p_list <- list()
for (i in seq_along(mat_list)) {
p_list[[paste0(names(mat_list)[[i]])]] <- ComplexHeatmap::Heatmap(
t(scale(t(mat_list[[i]]))),
#column_order=row.names(DEP_TMT_BL_Res@colData[order(DEP_TMT_BL_Res@colData$clid),]),
top_annotation = annotation_col,
column_split = factor(DEP_TMT_BL_Res@colData$clid),
name = " ",
column_title = paste0(names(mat_list)[i],"_TVG"),
show_column_names = F,
show_row_names = T
)
print(p_list[[i]])
}
# Save Heatmap
for (i in seq_along(p_list)) {
pdf(file=paste0(graph_dir_Heatmap_TVG, "CHeatmapLabel_" , names(p_list)[[i]], ".pdf"))
draw(p_list[[i]])
dev.off()
}
# Graphics termination
if(!is.null(dev.list())) dev.off()
## null device
## 1
if(!is.null(dev.list())) graphics.off()
# Directories
dir.create(paste0(output_dir,"Limma_res"))
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# comps
comps_factor <- as.vector(factor(paste0(colData(DEP_TMT_BL_Res)$condition)))
comps_df <- crossing(comp_num=comps_factor, comp_denom=comps_factor)
comps_df <- subset(comps_df, as.character(comps_df$comp_num) != as.character(comps_df$comp_denom))
# All comps
comps_df_all <- comps_df
comps_df_all <- cbind(comp_type="condition",comps_df_all)
comps_df_all <- cbind(comp_no=1:nrow(comps_df_all),comps_df_all)
comps_df_all$comp_name <- paste(comps_df_all$comp_num, "vs", comps_df_all$comp_denom, sep = "-")
comps_df_all$comp <- paste(comps_df_all$comp_num,comps_df_all$comp_denom, sep="-")
write.csv(comps_df_all, file=paste0(output_dir_res,"comps_all.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
comps_all_subset <- comps_df_all[,2:4]
# Unique comps only (for only unique combo)
comps_df_unique <- comps_df[!duplicated(t(apply(comps_df, 1, sort))), ]
comps_df_unique <- cbind(comp_type="condition",comps_df_unique )
comps_df_unique <- cbind(comp_no=1:nrow(comps_df_unique),comps_df_unique)
comps_df_unique$comp_name <- paste(comps_df_unique$comp_num, "vs", comps_df_unique$comp_denom, sep = "-")
comps_df_unique$comp <- paste(comps_df_unique$comp_num,comps_df_unique$comp_denom, sep="-")
write.csv(comps_df_unique, file=paste0(output_dir_res,"comps_unique.csv"), col.names = T, row.names = F)
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
comps_unique_subset <- comps_df_unique[,2:4]
# Comp list to use (all comps)
comps <- comps_df_all
# Extract
# Results
# mibs_comp_list
# Directories
dir.create(paste0(output_dir,"Limma_res"))
## Warning in dir.create(paste0(output_dir, "Limma_res")):
## '/Data/Output/MIB-DYN/DEP_TMT_BL_Res/Limma_res' already exists
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
## Warning in dir.create(paste0(graph_dir, "Limma_res")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_BL_Res/Limma_res' already exists
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# Extract results
DEP_TMT_limmaRes <- list()
for (i in seq_along(comps$comp)){
DEP_TMT_limmaRes[[comps$comp[i]]] <-
mibs_comp_list[[paste0(comps$comp[i])]]
}
# save results
for (i in seq_along(DEP_TMT_limmaRes)) {
write.csv(
DEP_TMT_limmaRes[[i]],
file = paste0(output_dir_res,"comp_", i, "_", names(DEP_TMT_limmaRes)[i], ".csv"),
row.names = TRUE
)
}
# Directories
dir.create(paste0(output_dir,"Limma_res"))
## Warning in dir.create(paste0(output_dir, "Limma_res")):
## '/Data/Output/MIB-DYN/DEP_TMT_BL_Res/Limma_res' already exists
output_dir_res <- paste0(output_dir,"Limma_res/")
dir.create(paste0(graph_dir,"Limma_res"))
## Warning in dir.create(paste0(graph_dir, "Limma_res")):
## '/Data/Graphs/MIB-DYN/DEP_TMT_BL_Res/Limma_res' already exists
graph_dir_res <- paste0(graph_dir,"Limma_res/")
# Extract comps of interest
coi <- comps_df_all[comps_df_all$comp_denom=="CEv3_0",]
comp_list_oi <- DEP_TMT_limmaRes[names(DEP_TMT_limmaRes) %in% coi$comp]
comp_list_oi <- lapply(comp_list_oi,data.frame)
# Label list of DF
for (i in seq_along(comp_list_oi)){
colnames(comp_list_oi[[i]]) <- paste0(colnames(comp_list_oi[[i]]),"_",names(comp_list_oi)[[i]])
comp_list_oi[[i]]$Gene_ID <- row.names(comp_list_oi[[i]])
}
# Left_join comps of interest to 1x spreadsheet
comp_list_oi_master <- Reduce(function(x, y) left_join(x, y, by="Gene_ID"), comp_list_oi)
row.names(comp_list_oi_master) <- comp_list_oi_master$Gene_ID
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)=="Gene_ID")]
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)%in% paste0(grep("gene_",colnames(comp_list_oi_master), value = T)))]
# Subset to spreadsheets
comp_list_oi_padj <- comp_list_oi_master[,grep("adj.P.Val_", colnames(comp_list_oi_master))]
comp_list_oi_pvalue <- comp_list_oi_master[,grep("P.Value_", colnames(comp_list_oi_master))]
comp_list_oi_l2fc <- comp_list_oi_master[,grep("logFC_", colnames(comp_list_oi_master))]
# Save spreadsheets
write.csv(comp_list_oi_padj, file = paste0(output_dir_res,"All_coi_","padj",".csv"))
write.csv(comp_list_oi_pvalue, file = paste0(output_dir_res,"All_coi_","pvalue",".csv"))
write.csv(comp_list_oi_l2fc, file = paste0(output_dir_res,"All_coi_","l2fc",".csv"))
# Directory for volcano plots
dir.create(paste0(graph_dir,"/","volcano"))
graph_dir_vol <- paste0(graph_dir,"volcano/")
# Plot Volcano
volcano_plots_ls <- list()
for (i in seq_along(DEP_TMT_limmaRes)){
plot(volcano_plots_ls[[names(DEP_TMT_limmaRes)[i]]] <-
EnhancedVolcano(
DEP_TMT_limmaRes[[i]],
lab=row.names(DEP_TMT_limmaRes[[i]]),
x="logFC",
y="adj.P.Val",
title= paste0("MIBs ",names(DEP_TMT_limmaRes)[[i]]),
pCutoff =qval,
FCcutoff =lfc,
pointSize = 2.0,
labSize = 3.0
))}
# Save Volcano
for (i in seq_along(volcano_plots_ls)){
ggsave(filename = paste0(graph_dir_vol,"comp_", i, "_", names(volcano_plots_ls)[[i]],".pdf"), plot=volcano_plots_ls[[i]])
}
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
# Graphics termination
if(!is.null(dev.list())) dev.off()
## null device
## 1
if(!is.null(dev.list())) graphics.off()
# Directories for DEP_TMT_EDA
dir.create(paste0(MIB_graphs,"DEP_TMT_EDA"))
dir.create(paste0(MIB_output,"DEP_TMT_EDA"))
graph_dir <- paste0(MIB_graphs,"DEP_TMT_EDA/")
output_dir <- paste0(MIB_output,"DEP_TMT_EDA/")
# Sample sheet
TMT_samples_all
## Prep Run Number Sample Cell.Line clid Timepoint serum Harvest
## 5 Prep_01 TMT02 6 P0298 C 701 0 starve 180807
## 6 Prep_01 TMT02 7 P0299 C 701 4 starve 180807
## 7 Prep_01 TMT02 8 P0300 C 701 24 starve 180808
## 8 Prep_01 TMT02 9 P0301 C 701 48 starve 180809
## 17 Prep_02 TMT05 21 P0294 CEv3 692 0 starve 180807
## 18 Prep_03 TMT05 22 P0295 CEv3 692 4 starve 180807
## 19 Prep_03 TMT05 23 P0296 CEv3 692 24 starve 180808
## 20 Prep_02 TMT05 24 P0297 CEv3 692 48 starve 180809
## 25 Prep_02 TMT07 31 P0117 C 701 0 starve 180705
## 26 Prep_02 TMT07 32 P0118 C 701 4 starve 180705
## 27 Prep_02 TMT07 33 P0119 C 701 24 starve 180706
## 28 Prep_02 TMT07 34 P0120 C 701 48 starve 180708
## 37 Prep_03 TMT10 46 P0290 CEv3 692 0 starve 180806
## 38 Prep_03 TMT10 47 P0291 CEv3 692 4 starve 180806
## 39 Prep_03 TMT10 48 P0292 CEv3 692 24 starve 180807
## 40 Prep_03 TMT10 49 P0293 CEv3 692 48 starve 180808
## 45 Prep_04 TMT12 56 P0113 C 701 0 starve 180704
## 46 Prep_04 TMT12 57 P0114 C 701 4 starve 180704
## 47 Prep_04 TMT12 58 P0115 C 701 24 starve 180705
## 48 Prep_04 TMT12 59 P0116 C 701 48 starve 180706
## 57 Prep_04 TMT15 71 P0286 CEv3 692 0 starve 180805
## 58 Prep_04 TMT15 72 P0287 CEv3 692 4 starve 180805
## 59 Prep_04 TMT15 73 P0288 CEv3 692 24 starve 180806
## 60 Prep_04 TMT15 74 P0289 CEv3 692 48 starve 180807
## 61 Prep_05 TMT16 76 P0368 G12 698 0 starve 180904
## 62 Prep_05 TMT16 77 P0369 G12 698 4 starve 180904
## 63 Prep_05 TMT16 78 P0370 G12 698 24 starve 180905
## 64 Prep_05 TMT16 79 P0371 G12 698 48 starve 180906
## 65 Prep_05 TMT17 81 P0356 G8 697 0 starve 180826
## 66 Prep_05 TMT17 82 P0357 G8 697 4 starve 180826
## 67 Prep_05 TMT17 83 P0358 G8 697 24 starve 180827
## 68 Prep_05 TMT17 84 P0359 G8 697 48 starve 180828
## 69 Prep_05 TMT18 86 P0344 G5 696 0 starve 180905
## 70 Prep_05 TMT18 87 P0345 G5 696 4 starve 180905
## 71 Prep_05 TMT18 88 P0346 G5 696 24 starve 180906
## 72 Prep_05 TMT18 89 P0347 G5 696 48 starve 180907
## 73 Prep_05 TMT19 91 P0332 G1 695 0 starve 180826
## 74 Prep_05 TMT19 92 P0333 G1 695 4 starve 180826
## 75 Prep_05 TMT19 93 P0334 G1 695 24 starve 180827
## 76 Prep_05 TMT19 94 P0335 G1 695 48 starve 180828
## 77 Prep_06 TMT20 96 P0204 E5 694 0 starve 180724
## 78 Prep_06 TMT20 97 P0205 E5 694 4 starve 180724
## 79 Prep_06 TMT20 98 P0206 E5 694 24 starve 180725
## 80 Prep_06 TMT20 99 P0207 E5 694 48 starve 180726
## 81 Prep_06 TMT21 101 P0189 E4 693 0 starve 180724
## 82 Prep_06 TMT21 102 P0190 E4 693 4 starve 180724
## 83 Prep_06 TMT21 103 P0191 E4 693 24 starve 180724
## 84 Prep_06 TMT21 104 P0192 E4 693 48 starve 180725
## 97 Prep_07 TMT25 121 P0364 G12 698 0 starve 180905
## 98 Prep_07 TMT25 122 P0365 G12 698 4 starve 180905
## 99 Prep_07 TMT25 123 P0366 G12 698 24 starve 180906
## 100 Prep_07 TMT25 124 P0367 G12 698 48 starve 180907
## 101 Prep_07 TMT26 126 P0352 G8 697 0 starve 180825
## 102 Prep_07 TMT26 127 P0353 G8 697 4 starve 180825
## 103 Prep_07 TMT26 128 P0354 G8 697 24 starve 180826
## 104 Prep_07 TMT26 129 P0355 G8 697 48 starve 180827
## 105 Prep_07 TMT27 131 P0340 G5 696 0 starve 180906
## 106 Prep_07 TMT27 132 P0341 G5 696 4 starve 180906
## 107 Prep_07 TMT27 133 P0342 G5 696 24 starve 180907
## 108 Prep_07 TMT27 134 P0343 G5 696 48 starve 180908
## 109 Prep_08 TMT28 136 P0328 G1 695 0 starve 180825
## 110 Prep_08 TMT28 137 P0329 G1 695 4 starve 180825
## 111 Prep_08 TMT28 138 P0330 G1 695 24 starve 180826
## 112 Prep_08 TMT28 139 P0331 G1 695 48 starve 180827
## 113 Prep_08 TMT29 141 P0200 E5 694 0 starve 180723
## 114 Prep_08 TMT29 142 P0201 E5 694 4 starve 180723
## 115 Prep_08 TMT29 143 P0202 E5 694 24 starve 180724
## 116 Prep_08 TMT29 144 P0203 E5 694 48 starve 180725
## 117 Prep_08 TMT30 146 P0185 E4 693 0 starve 180723
## 118 Prep_08 TMT30 147 P0186 E4 693 4 starve 180723
## 119 Prep_08 TMT30 148 P0187 E4 693 24 starve 180724
## 120 Prep_08 TMT30 149 P0188 E4 693 48 starve 180725
## 133 Prep_09 TMT34 166 P0360 G12 698 0 starve 180826
## 134 Prep_09 TMT34 167 P0361 G12 698 4 starve 180826
## 135 Prep_09 TMT34 168 P0362 G12 698 24 starve 180827
## 136 Prep_09 TMT34 169 P0363 G12 698 48 starve 180828
## 137 Prep_10 TMT35 171 P0348 G8 697 0 starve 180824
## 138 Prep_10 TMT35 172 P0349 G8 697 4 starve 180824
## 139 Prep_10 TMT35 173 P0350 G8 697 24 starve 180825
## 140 Prep_10 TMT35 174 P0351 G8 697 48 starve 180826
## 141 Prep_10 TMT36 176 P0336 G5 696 0 starve 180826
## 142 Prep_10 TMT36 177 P0337 G5 696 4 starve 180826
## 143 Prep_10 TMT36 178 P0338 G5 696 24 starve 180827
## 144 Prep_10 TMT36 179 P0339 G5 696 48 starve 180828
## 145 Prep_10 TMT37 181 P0324 G1 695 0 starve 180824
## 146 Prep_10 TMT37 182 P0325 G1 695 4 starve 180824
## 147 Prep_10 TMT37 183 P0326 G1 695 24 starve 180825
## 148 Prep_10 TMT37 184 P0327 G1 695 48 starve 180826
## 149 Prep_10 TMT38 186 P0196 E5 694 0 starve 180722
## 150 Prep_10 TMT38 187 P0197 E5 694 4 starve 180722
## 151 Prep_10 TMT38 188 P0198 E5 694 24 starve 180723
## 152 Prep_10 TMT38 189 P0199 E5 694 48 starve 180724
## 153 Prep_11 TMT39 191 P0181 E4 693 0 starve 180722
## 154 Prep_11 TMT39 192 P0182 E4 693 4 starve 180722
## 155 Prep_11 TMT39 193 P0183 E4 693 24 starve 180723
## 156 Prep_11 TMT39 194 P0184 E4 693 48 starve 180724
## Label_num Tag Exp_rep TMT.Reporter label condition replicate name
## 5 126 R1 3 TMT02_R1 TMT02_R1 C_0 3 C_0_3
## 6 127 R2 3 TMT02_R2 TMT02_R2 C_4 3 C_4_3
## 7 128 R3 3 TMT02_R3 TMT02_R3 C_24 3 C_24_3
## 8 129 R4 3 TMT02_R4 TMT02_R4 C_48 3 C_48_3
## 17 126 R1 3 TMT05_R1 TMT05_R1 CEv3_0 3 CEv3_0_3
## 18 127 R2 3 TMT05_R2 TMT05_R2 CEv3_4 3 CEv3_4_3
## 19 128 R3 3 TMT05_R3 TMT05_R3 CEv3_24 3 CEv3_24_3
## 20 129 R4 3 TMT05_R4 TMT05_R4 CEv3_48 3 CEv3_48_3
## 25 126 R1 2 TMT07_R1 TMT07_R1 C_0 2 C_0_2
## 26 127 R2 2 TMT07_R2 TMT07_R2 C_4 2 C_4_2
## 27 128 R3 2 TMT07_R3 TMT07_R3 C_24 2 C_24_2
## 28 129 R4 2 TMT07_R4 TMT07_R4 C_48 2 C_48_2
## 37 126 R1 2 TMT10_R1 TMT10_R1 CEv3_0 2 CEv3_0_2
## 38 127 R2 2 TMT10_R2 TMT10_R2 CEv3_4 2 CEv3_4_2
## 39 128 R3 2 TMT10_R3 TMT10_R3 CEv3_24 2 CEv3_24_2
## 40 129 R4 2 TMT10_R4 TMT10_R4 CEv3_48 2 CEv3_48_2
## 45 126 R1 1 TMT12_R1 TMT12_R1 C_0 1 C_0_1
## 46 127 R2 1 TMT12_R2 TMT12_R2 C_4 1 C_4_1
## 47 128 R3 1 TMT12_R3 TMT12_R3 C_24 1 C_24_1
## 48 129 R4 1 TMT12_R4 TMT12_R4 C_48 1 C_48_1
## 57 126 R1 1 TMT15_R1 TMT15_R1 CEv3_0 1 CEv3_0_1
## 58 127 R2 1 TMT15_R2 TMT15_R2 CEv3_4 1 CEv3_4_1
## 59 128 R3 1 TMT15_R3 TMT15_R3 CEv3_24 1 CEv3_24_1
## 60 129 R4 1 TMT15_R4 TMT15_R4 CEv3_48 1 CEv3_48_1
## 61 126 R1 3 TMT16_R1 TMT16_R1 G12_0 3 G12_0_3
## 62 127 R2 3 TMT16_R2 TMT16_R2 G12_4 3 G12_4_3
## 63 128 R3 3 TMT16_R3 TMT16_R3 G12_24 3 G12_24_3
## 64 129 R4 3 TMT16_R4 TMT16_R4 G12_48 3 G12_48_3
## 65 126 R1 3 TMT17_R1 TMT17_R1 G8_0 3 G8_0_3
## 66 127 R2 3 TMT17_R2 TMT17_R2 G8_4 3 G8_4_3
## 67 128 R3 3 TMT17_R3 TMT17_R3 G8_24 3 G8_24_3
## 68 129 R4 3 TMT17_R4 TMT17_R4 G8_48 3 G8_48_3
## 69 126 R1 3 TMT18_R1 TMT18_R1 G5_0 3 G5_0_3
## 70 127 R2 3 TMT18_R2 TMT18_R2 G5_4 3 G5_4_3
## 71 128 R3 3 TMT18_R3 TMT18_R3 G5_24 3 G5_24_3
## 72 129 R4 3 TMT18_R4 TMT18_R4 G5_48 3 G5_48_3
## 73 126 R1 3 TMT19_R1 TMT19_R1 G1_0 3 G1_0_3
## 74 127 R2 3 TMT19_R2 TMT19_R2 G1_4 3 G1_4_3
## 75 128 R3 3 TMT19_R3 TMT19_R3 G1_24 3 G1_24_3
## 76 129 R4 3 TMT19_R4 TMT19_R4 G1_48 3 G1_48_3
## 77 126 R1 3 TMT20_R1 TMT20_R1 E5_0 3 E5_0_3
## 78 127 R2 3 TMT20_R2 TMT20_R2 E5_4 3 E5_4_3
## 79 128 R3 3 TMT20_R3 TMT20_R3 E5_24 3 E5_24_3
## 80 129 R4 3 TMT20_R4 TMT20_R4 E5_48 3 E5_48_3
## 81 126 R1 3 TMT21_R1 TMT21_R1 E4_0 3 E4_0_3
## 82 127 R2 3 TMT21_R2 TMT21_R2 E4_4 3 E4_4_3
## 83 128 R3 3 TMT21_R3 TMT21_R3 E4_24 3 E4_24_3
## 84 129 R4 3 TMT21_R4 TMT21_R4 E4_48 3 E4_48_3
## 97 126 R1 2 TMT25_R1 TMT25_R1 G12_0 2 G12_0_2
## 98 127 R2 2 TMT25_R2 TMT25_R2 G12_4 2 G12_4_2
## 99 128 R3 2 TMT25_R3 TMT25_R3 G12_24 2 G12_24_2
## 100 129 R4 2 TMT25_R4 TMT25_R4 G12_48 2 G12_48_2
## 101 126 R1 2 TMT26_R1 TMT26_R1 G8_0 2 G8_0_2
## 102 127 R2 2 TMT26_R2 TMT26_R2 G8_4 2 G8_4_2
## 103 128 R3 2 TMT26_R3 TMT26_R3 G8_24 2 G8_24_2
## 104 129 R4 2 TMT26_R4 TMT26_R4 G8_48 2 G8_48_2
## 105 126 R1 2 TMT27_R1 TMT27_R1 G5_0 2 G5_0_2
## 106 127 R2 2 TMT27_R2 TMT27_R2 G5_4 2 G5_4_2
## 107 128 R3 2 TMT27_R3 TMT27_R3 G5_24 2 G5_24_2
## 108 129 R4 2 TMT27_R4 TMT27_R4 G5_48 2 G5_48_2
## 109 126 R1 2 TMT28_R1 TMT28_R1 G1_0 2 G1_0_2
## 110 127 R2 2 TMT28_R2 TMT28_R2 G1_4 2 G1_4_2
## 111 128 R3 2 TMT28_R3 TMT28_R3 G1_24 2 G1_24_2
## 112 129 R4 2 TMT28_R4 TMT28_R4 G1_48 2 G1_48_2
## 113 126 R1 2 TMT29_R1 TMT29_R1 E5_0 2 E5_0_2
## 114 127 R2 2 TMT29_R2 TMT29_R2 E5_4 2 E5_4_2
## 115 128 R3 2 TMT29_R3 TMT29_R3 E5_24 2 E5_24_2
## 116 129 R4 2 TMT29_R4 TMT29_R4 E5_48 2 E5_48_2
## 117 126 R1 2 TMT30_R1 TMT30_R1 E4_0 2 E4_0_2
## 118 127 R2 2 TMT30_R2 TMT30_R2 E4_4 2 E4_4_2
## 119 128 R3 2 TMT30_R3 TMT30_R3 E4_24 2 E4_24_2
## 120 129 R4 2 TMT30_R4 TMT30_R4 E4_48 2 E4_48_2
## 133 126 R1 1 TMT34_R1 TMT34_R1 G12_0 1 G12_0_1
## 134 127 R2 1 TMT34_R2 TMT34_R2 G12_4 1 G12_4_1
## 135 128 R3 1 TMT34_R3 TMT34_R3 G12_24 1 G12_24_1
## 136 129 R4 1 TMT34_R4 TMT34_R4 G12_48 1 G12_48_1
## 137 126 R1 1 TMT35_R1 TMT35_R1 G8_0 1 G8_0_1
## 138 127 R2 1 TMT35_R2 TMT35_R2 G8_4 1 G8_4_1
## 139 128 R3 1 TMT35_R3 TMT35_R3 G8_24 1 G8_24_1
## 140 129 R4 1 TMT35_R4 TMT35_R4 G8_48 1 G8_48_1
## 141 126 R1 1 TMT36_R1 TMT36_R1 G5_0 1 G5_0_1
## 142 127 R2 1 TMT36_R2 TMT36_R2 G5_4 1 G5_4_1
## 143 128 R3 1 TMT36_R3 TMT36_R3 G5_24 1 G5_24_1
## 144 129 R4 1 TMT36_R4 TMT36_R4 G5_48 1 G5_48_1
## 145 126 R1 1 TMT37_R1 TMT37_R1 G1_0 1 G1_0_1
## 146 127 R2 1 TMT37_R2 TMT37_R2 G1_4 1 G1_4_1
## 147 128 R3 1 TMT37_R3 TMT37_R3 G1_24 1 G1_24_1
## 148 129 R4 1 TMT37_R4 TMT37_R4 G1_48 1 G1_48_1
## 149 126 R1 1 TMT38_R1 TMT38_R1 E5_0 1 E5_0_1
## 150 127 R2 1 TMT38_R2 TMT38_R2 E5_4 1 E5_4_1
## 151 128 R3 1 TMT38_R3 TMT38_R3 E5_24 1 E5_24_1
## 152 129 R4 1 TMT38_R4 TMT38_R4 E5_48 1 E5_48_1
## 153 126 R1 1 TMT39_R1 TMT39_R1 E4_0 1 E4_0_1
## 154 127 R2 1 TMT39_R2 TMT39_R2 E4_4 1 E4_4_1
## 155 128 R3 1 TMT39_R3 TMT39_R3 E4_24 1 E4_24_1
## 156 129 R4 1 TMT39_R4 TMT39_R4 E4_48 1 E4_48_1
## EGFR_TKI_sen measure
## 5 Sensitive Intensity
## 6 Sensitive Intensity
## 7 Sensitive Intensity
## 8 Sensitive Intensity
## 17 Sensitive Intensity
## 18 Sensitive Intensity
## 19 Sensitive Intensity
## 20 Sensitive Intensity
## 25 Sensitive Intensity
## 26 Sensitive Intensity
## 27 Sensitive Intensity
## 28 Sensitive Intensity
## 37 Sensitive Intensity
## 38 Sensitive Intensity
## 39 Sensitive Intensity
## 40 Sensitive Intensity
## 45 Sensitive Intensity
## 46 Sensitive Intensity
## 47 Sensitive Intensity
## 48 Sensitive Intensity
## 57 Sensitive Intensity
## 58 Sensitive Intensity
## 59 Sensitive Intensity
## 60 Sensitive Intensity
## 61 Resistant Intensity
## 62 Resistant Intensity
## 63 Resistant Intensity
## 64 Resistant Intensity
## 65 Resistant Intensity
## 66 Resistant Intensity
## 67 Resistant Intensity
## 68 Resistant Intensity
## 69 Resistant Intensity
## 70 Resistant Intensity
## 71 Resistant Intensity
## 72 Resistant Intensity
## 73 Resistant Intensity
## 74 Resistant Intensity
## 75 Resistant Intensity
## 76 Resistant Intensity
## 77 Resistant Intensity
## 78 Resistant Intensity
## 79 Resistant Intensity
## 80 Resistant Intensity
## 81 Resistant Intensity
## 82 Resistant Intensity
## 83 Resistant Intensity
## 84 Resistant Intensity
## 97 Resistant Intensity
## 98 Resistant Intensity
## 99 Resistant Intensity
## 100 Resistant Intensity
## 101 Resistant Intensity
## 102 Resistant Intensity
## 103 Resistant Intensity
## 104 Resistant Intensity
## 105 Resistant Intensity
## 106 Resistant Intensity
## 107 Resistant Intensity
## 108 Resistant Intensity
## 109 Resistant Intensity
## 110 Resistant Intensity
## 111 Resistant Intensity
## 112 Resistant Intensity
## 113 Resistant Intensity
## 114 Resistant Intensity
## 115 Resistant Intensity
## 116 Resistant Intensity
## 117 Resistant Intensity
## 118 Resistant Intensity
## 119 Resistant Intensity
## 120 Resistant Intensity
## 133 Resistant Intensity
## 134 Resistant Intensity
## 135 Resistant Intensity
## 136 Resistant Intensity
## 137 Resistant Intensity
## 138 Resistant Intensity
## 139 Resistant Intensity
## 140 Resistant Intensity
## 141 Resistant Intensity
## 142 Resistant Intensity
## 143 Resistant Intensity
## 144 Resistant Intensity
## 145 Resistant Intensity
## 146 Resistant Intensity
## 147 Resistant Intensity
## 148 Resistant Intensity
## 149 Resistant Intensity
## 150 Resistant Intensity
## 151 Resistant Intensity
## 152 Resistant Intensity
## 153 Resistant Intensity
## 154 Resistant Intensity
## 155 Resistant Intensity
## 156 Resistant Intensity
# Subset by cell line
TMT_samples_EDA_ls <- list()
for ( i in unique(TMT_samples_all$Cell.Line)){
TMT_samples_EDA_ls[[i]] <-
subset(TMT_samples_all, TMT_samples_all$Cell.Line==paste0(i))
}
# Add additional groups
TMT_samples_EDA_ls <- c(TMT_samples_EDA_ls,
list(
TMT_samples_BL_Res=TMT_samples_BL_Res,
TMT_samples_Res=TMT_samples_Res,
TMT_samples_Par=TMT_samples_Par
))
# Save sample sheet
for (i in seq_along(TMT_samples_EDA_ls)){
dir.create(paste0(graph_dir,names(TMT_samples_EDA_ls)[i]))
dir.create(paste0(output_dir,names(TMT_samples_EDA_ls)[i]))
write.csv(TMT_samples_EDA_ls[[i]], file=paste0(output_dir,names(TMT_samples_EDA_ls)[i],"/TMT_samples",".csv"))
}
# Imputed values for TMT
se_mibs_TMT_mv
## class: SummarizedExperiment
## dim: 306 96
## metadata(0):
## assays(1): ''
## rownames(306): Ephb6 Clk2 ... Riok3 Pkmyt1
## rowData names(4): ID name imputed num_NAs
## colnames(96): C_0_3 C_4_3 ... E4_24_1 E4_48_1
## colData names(20): Prep Run ... EGFR_TKI_sen measure
# Save as RDS
saveRDS(se_mibs_TMT_mv, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","se_mibs_TMT_mv",".rds"))
# Batch corrected values for TMT
se_mibs_TMT_bc
## class: SummarizedExperiment
## dim: 306 96
## metadata(0):
## assays(1): ''
## rownames(306): Ephb6 Clk2 ... Riok3 Pkmyt1
## rowData names(4): ID name imputed num_NAs
## colnames(96): C_0_3 C_4_3 ... E4_24_1 E4_48_1
## colData names(20): Prep Run ... EGFR_TKI_sen measure
# Save as RDS
saveRDS(se_mibs_TMT_bc, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","se_mibs_TMT_bc",".rds"))
# Generate se subsets for DEP EDA
DEP_TMT_EDA_mv_ls <- list()
DEP_TMT_EDA_bc_ls <- list()
for (i in seq_along(TMT_samples_EDA_ls)){
# Generate se object for imputed missing values
DEP_TMT_EDA_mv_ls[[names(TMT_samples_EDA_ls)[i]]] <-
se_mibs_TMT_mv[,paste0(TMT_samples_EDA_ls[[i]]$condition,"_",TMT_samples_EDA_ls[[i]]$replicate)]
# Save as RDS
saveRDS(DEP_TMT_EDA_mv_ls[[names(TMT_samples_EDA_ls)[i]]], file = paste0(output_dir,names(TMT_samples_EDA_ls)[i],"/",format(Sys.Date(),"%y%m%d"),"_",names(TMT_samples_EDA_ls)[i],"_mv_",".rds"))
# Generate se object for batch corrected values
DEP_TMT_EDA_bc_ls[[names(TMT_samples_EDA_ls)[i]]] <-
se_mibs_TMT_bc[,paste0(TMT_samples_EDA_ls[[i]]$condition,"_",TMT_samples_EDA_ls[[i]]$replicate)]
# Save as RDS
saveRDS(DEP_TMT_EDA_bc_ls[[names(TMT_samples_EDA_ls)[i]]], file = paste0(output_dir,names(TMT_samples_EDA_ls)[i],"/",format(Sys.Date(),"%y%m%d"),"_",names(TMT_samples_EDA_ls)[i],"_bc_",".rds"))
# Save imputed(raw) and batch corrected (norm) norm counts
norm_counts <- as.data.frame(assay(DEP_TMT_EDA_bc_ls[[names(TMT_samples_EDA_ls)[i]]]))
raw_counts <- as.data.frame(assay(DEP_TMT_EDA_mv_ls[[names(TMT_samples_EDA_ls)[i]]]))
write.csv(
norm_counts,
file=paste0(output_dir,names(TMT_samples_EDA_ls)[i],"/","norm_counts",".csv"),
row.names = TRUE
)
write.csv(
raw_counts,
file=paste0(output_dir,names(TMT_samples_EDA_ls)[i],"/","raw_counts",".csv"),
row.names = TRUE
)
}
# Save as RDS
saveRDS(DEP_TMT_EDA_mv_ls, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","DEP_TMT_EDA_mv_ls",".rds"))
# Save as RDS
saveRDS(DEP_TMT_EDA_bc_ls, file = paste0(output_dir,format(Sys.Date(),"%y%m%d"),"_","DEP_TMT_EDA_bc_ls",".rds"))
# PCA with all genes
# BC PCA
for(h in seq_along(DEP_TMT_EDA_bc_ls)){
# Directories
PCA_graph <- paste0(graph_dir,names(DEP_TMT_EDA_bc_ls)[h],"/")
PCA_output <- paste0(output_dir,names(DEP_TMT_EDA_bc_ls)[h],"/")
# Extract single
DEP_bc <- DEP_TMT_EDA_bc_ls[[h]]
# Create pca object
pcaData <- PCAtools::pca(assay(DEP_bc), metadata = colData(DEP_bc))
# Save pca data
write.csv(pcaData$rotated, file =paste0(PCA_output,"pcaData","DEP_bc",".csv"))
# Set colors
color_vector <-
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Timepoint',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
pairsplot
# save graph as PDF
ggsave(
filename = paste0(PCA_graph,"DEP_bc_","pairsplot", ".pdf"),
plot = pairsplot
)
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Timepoint",
colkey= color_vector,
legendPosition = 'right' )
p1
# save graph as PDF
ggsave(
filename = paste0(PCA_graph,"pca_DEP_bc_","Timepoint",".pdf"),
plot = p1
)
}
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
# Missing Values PCA
for(h in seq_along(DEP_TMT_EDA_mv_ls)){
# Directories
PCA_graph <- paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[h],"/")
PCA_output <- paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[h],"/")
# Extract single
DEP_mv <- DEP_TMT_EDA_mv_ls[[h]]
# Create pca object
pcaData <- PCAtools::pca(assay(DEP_mv), metadata = colData(DEP_mv))
# Save pca data
write.csv(pcaData$rotated, file =paste0(PCA_output,"pcaData","DEP_mv",".csv"))
# Set colors
color_vector <-
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
)
# plot 5 pricipal components in a pairsplot
pairsplot <- pairsplot(pcaData,
components = getComponents(pcaData, c(1:5)),
triangle = TRUE, trianglelabSize = 12,
hline = 0, vline = 0,
pointSize = 0.4,
gridlines.major = FALSE, gridlines.minor = FALSE,
colby = 'Timepoint',
colkey= color_vector,
title = 'Pairs plot', plotaxes = FALSE,
legendPosition = 'none',
margingaps = unit(c(-0.01, -0.01, -0.01, -0.01), 'cm'))
pairsplot
# save graph as PDF
ggsave(
filename = paste0(PCA_graph,"DEP_mv","pairsplot", ".pdf"),
plot = pairsplot
)
# PCA 1
p1 <- biplot(pcaData, x = "PC1", y = "PC2",
lab = NULL ,
colby = "Timepoint",
colkey= color_vector,
legendPosition = 'right' )
p1
# save graph as PDF
ggsave(
filename = paste0(PCA_graph,"pca_DEP_mv_","Timepoint",".pdf"),
plot = p1
)
}
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Coordinate system already present. Adding new coordinate system, which will
## replace the existing one.
## Saving 7 x 5 in image
## Scale for colour is already present.
## Adding another scale for colour, which will replace the existing scale.
## Saving 7 x 5 in image
Batch corrected values
system.time(
for(h in seq_along(DEP_TMT_EDA_bc_ls)){
# Directories
Euclidian_graph <- paste0(graph_dir,names(DEP_TMT_EDA_bc_ls)[h],"/")
Euclidian_output <- paste0(output_dir,names(DEP_TMT_EDA_bc_ls)[h],"/")
# Extract single
DEP_bc <- DEP_TMT_EDA_bc_ls[[h]]
# calculate sample euclidian distances
sampleDists <- dist(t(assay(DEP_bc)))
# plot sample euclidian distances via a heatmap
sampleDistMatrix <- as.matrix( sampleDists )
# Complex Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_bc@colData$Cell.Line,
Time = DEP_bc@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
)
))
# Heatmap of euclidian distances
ed_heatmap <-
ComplexHeatmap::Heatmap(
sampleDistMatrix,
name = " ",
column_title = "Euclidian Distances",
show_column_names = FALSE,
show_row_names = FALSE,
clustering_distance_rows = "euclidean",
clustering_distance_columns = "euclidean",
clustering_method_rows = "complete",
clustering_method_columns = "complete",
col = colorRampPalette( rev(brewer.pal(9, "Blues")) )(255),
top_annotation = annotation_col
)
pdf(file=paste0(Euclidian_graph,"Euclidian_distance_bc",".pdf"))
draw(ed_heatmap)
dev.off()
}
)
## user system elapsed
## 2.574 0.000 2.668
Missing Values
system.time(
for(h in seq_along(DEP_TMT_EDA_mv_ls)){
# Directories
Euclidian_graph <- paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[h],"/")
Euclidian_output <- paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[h],"/")
# Extract single
DEP_mv <- DEP_TMT_EDA_mv_ls[[h]]
# calculate sample euclidian distances
sampleDists <- dist(t(assay(DEP_mv)))
# plot sample euclidian distances via a heatmap
sampleDistMatrix <- as.matrix( sampleDists )
# Complex Heatmap annotation labels
annotation_col<-
HeatmapAnnotation(df = data.frame(Model = DEP_mv@colData$Cell.Line,
Time = DEP_mv@colData$Timepoint),
col = list(Model =
c(
'C' = 'black',
'CEv3' = 'red',
'E4' = 'deepskyblue4',
'E5' = 'turquoise3',
'G1' = 'purple4',
'G5' = 'mediumorchid',
'G8' = 'deeppink',
'G12' = 'pink2'
),
Time =
c(
'0' = 'black',
'4' = 'purple',
'24' = 'darkgreen',
'48' = 'darkorange'
)
))
# Heatmap of euclidian distances
ed_heatmap <-
ComplexHeatmap::Heatmap(
sampleDistMatrix,
name = " ",
column_title = "Euclidian Distances",
show_column_names = FALSE,
show_row_names = FALSE,
clustering_distance_rows = "euclidean",
clustering_distance_columns = "euclidean",
clustering_method_rows = "complete",
clustering_method_columns = "complete",
col = colorRampPalette( rev(brewer.pal(9, "Blues")) )(255),
top_annotation = annotation_col
)
pdf(file=paste0(Euclidian_graph,"Euclidian_distance_mv",".pdf"))
draw(ed_heatmap)
dev.off()
}
)
## user system elapsed
## 2.615 0.010 2.911
This chunk will extract the normalized batch corrected counts for GOI ##### normCounts
# user system elapsed
# 602.754 5.045 732.333
system.time(for (h in seq_along(DEP_TMT_EDA_bc_ls)){
# Directories for normCounts
dir.create(paste0(output_dir,names(DEP_TMT_EDA_bc_ls)[h],"/","normCounts"))
output_dir_normCounts <- paste0(output_dir,names(DEP_TMT_EDA_bc_ls)[h],"/","normCounts/")
dir.create(paste0(graph_dir,names(DEP_TMT_EDA_bc_ls)[h],"/","normCounts"))
graph_dir_normCounts <- paste0(graph_dir,names(DEP_TMT_EDA_bc_ls)[h],"/","normCounts/")
DEP_normCounts <- DEP_TMT_EDA_bc_ls[[h]]
# DEP_normCounts
norm_counts <- as.data.frame(assay(DEP_normCounts))
normCounts <- norm_counts
# Import DF of GOI
# normCounts_goi_df <- read.csv("/Data/Input/RNAseq/REF/normCounts_goi_mouse.csv",header = TRUE, fileEncoding = "UTF-8-BOM")
# vectors of genes of interest. Filtered for present
goi <- row.names(DEP_normCounts)
# Check all goi are present after filtering
match(goi,row.names(normCounts))
# Transform data
counts_goi <- as.data.frame(t(normCounts[goi,]))
counts.rn_counts_goi <- rownames(counts_goi)
# Extract each gene in to a unique dataframe
counts_data_list_goi <- list()
for (i in seq_along(goi)) {
counts_data_list_goi[[goi[i]]] <- as.data.frame(counts_goi[, i])
rownames(counts_data_list_goi[[goi[i]]]) <- counts.rn_counts_goi
counts_data_list_goi[[goi[i]]] <-
rownames_to_column(counts_data_list_goi[[goi[i]]], var = "sample_id")
colnames(counts_data_list_goi[[goi[i]]])[2] <- "norm_counts"
counts_data_list_goi[[goi[i]]]$cell_line_abbr <-
DEP_normCounts@colData$Cell.Line[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$drug <-
DEP_normCounts@colData$drug[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$drug_conc_uM <-
DEP_normCounts@colData$drug_conc_uM[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$time_h <-
DEP_normCounts@colData$Timepoint[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$condition <-
DEP_normCounts@colData$condition[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
}
# Save count data for GOI
for (i in seq_along(counts_data_list_goi)) {
write.csv(
counts_data_list_goi[[i]],
file = paste0(output_dir_normCounts, names(counts_data_list_goi)[i], ".csv"),
row.names = TRUE
)
}
# Compute stats for GOI
counts_data_list_goi_stats <- list()
for (i in seq_along(counts_data_list_goi)) {
counts_data_list_goi_stats[[names(counts_data_list_goi)[i]]] <-
counts_data_list_goi[[i]] %>%
group_by(time_h) %>%
summarise(
n = n(),
avg_normCount = mean(norm_counts),
sd = sd(norm_counts),
IQR = IQR(norm_counts),
MAD = mad(norm_counts),
Coeff.Variation.Prcnt =sd(norm_counts) / mean(norm_counts) * 100
)
}
# Save stats data for GOI
for (i in seq_along(counts_data_list_goi_stats)) {
write.csv(
counts_data_list_goi_stats[[i]],
file = paste0(output_dir_normCounts, names(counts_data_list_goi_stats)[i], "_stats.csv"),
row.names = TRUE
)
}
# Boxplots on linear scale
b <- list()
for (i in seq_along(counts_data_list_goi)) {
b[[i]] <- ggplot(data = counts_data_list_goi[[i]],
aes(x = factor(counts_data_list_goi[[i]]$condition, levels = mixedsort(unique(counts_data_list_goi[[i]]$condition))),
y = counts_data_list_goi[[i]]$norm_counts)) +
geom_boxplot() +
geom_point() +
xlab("Genotype") +
ylab("Normalized Counts") +
scale_y_continuous() +
labs(title = paste0(names(counts_data_list_goi[i]), " DEP_normCounts")) +
theme(axis.text.x = element_text(angle = 90, hjust = 0.5, vjust = 0.5, size=rel(1)))
print(b[[i]])
ggsave(filename = paste0(
graph_dir_normCounts,
names(counts_data_list_goi)[[i]],
"_normCounts",
".pdf"
))
}
# Boxplots on linear scale
b <- list()
for (i in seq_along(counts_data_list_goi)) {
b[[i]] <- ggplot(data = counts_data_list_goi[[i]],
aes(x = counts_data_list_goi[[i]]$condition ,
y = counts_data_list_goi[[i]]$norm_counts)) +
geom_boxplot() +
geom_point() +
xlab("Genotype") +
ylab("Normalized Counts") +
scale_y_continuous() +
labs(title = paste0(names(counts_data_list_goi[i]), " DEP_normCounts")) +
theme(axis.text.x = element_text(angle = 90, hjust = 0.5, vjust = 0.5, size=rel(1)))
print(b[[i]])
ggsave(filename = paste0(
graph_dir_normCounts,
names(counts_data_list_goi)[[i]],
"_normCounts",
".pdf"
))
}
# Graphics termination
if(!is.null(dev.list())) dev.off()
if(!is.null(dev.list())) graphics.off()
})
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## user system elapsed
## 987.934 2.991 1074.034
This chunk will extract the Impute counts for GOI ##### normCounts (MV)
# user system elapsed
# 602.754 5.045 732.333
system.time(for (h in seq_along(DEP_TMT_EDA_mv_ls)){
# Directories for normCounts
dir.create(paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[h],"/","normCounts_MV"))
output_dir_normCounts <- paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[h],"/","normCounts_MV/")
dir.create(paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[h],"/","normCounts_MV"))
graph_dir_normCounts <- paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[h],"/","normCounts_MV/")
DEP_normCounts <- DEP_TMT_EDA_mv_ls[[h]]
# DEP_normCounts
norm_counts <- as.data.frame(assay(DEP_normCounts))
normCounts <- norm_counts
# Import DF of GOI
# normCounts_goi_df <- read.csv("/Data/Input/RNAseq/REF/normCounts_goi_mouse.csv",header = TRUE, fileEncoding = "UTF-8-BOM")
# vectors of genes of interest. Filtered for present
goi <- row.names(DEP_normCounts)
# Check all goi are present after filtering
match(goi,row.names(normCounts))
# Transform data
counts_goi <- as.data.frame(t(normCounts[goi,]))
counts.rn_counts_goi <- rownames(counts_goi)
# Extract each gene in to a unique dataframe
counts_data_list_goi <- list()
for (i in seq_along(goi)) {
counts_data_list_goi[[goi[i]]] <- as.data.frame(counts_goi[, i])
rownames(counts_data_list_goi[[goi[i]]]) <- counts.rn_counts_goi
counts_data_list_goi[[goi[i]]] <-
rownames_to_column(counts_data_list_goi[[goi[i]]], var = "sample_id")
colnames(counts_data_list_goi[[goi[i]]])[2] <- "norm_counts"
counts_data_list_goi[[goi[i]]]$cell_line_abbr <-
DEP_normCounts@colData$Cell.Line[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$drug <-
DEP_normCounts@colData$drug[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$drug_conc_uM <-
DEP_normCounts@colData$drug_conc_uM[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$time_h <-
DEP_normCounts@colData$Timepoint[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
counts_data_list_goi[[goi[i]]]$condition <-
DEP_normCounts@colData$condition[match(counts_data_list_goi[[goi[i]]]$sample_id,
rownames(DEP_normCounts@colData))]
}
# Save count data for GOI
for (i in seq_along(counts_data_list_goi)) {
write.csv(
counts_data_list_goi[[i]],
file = paste0(output_dir_normCounts, names(counts_data_list_goi)[i], ".csv"),
row.names = TRUE
)
}
# Compute stats for GOI
counts_data_list_goi_stats <- list()
for (i in seq_along(counts_data_list_goi)) {
counts_data_list_goi_stats[[names(counts_data_list_goi)[i]]] <-
counts_data_list_goi[[i]] %>%
group_by(time_h) %>%
summarise(
n = n(),
avg_normCount = mean(norm_counts),
sd = sd(norm_counts),
IQR = IQR(norm_counts),
MAD = mad(norm_counts),
Coeff.Variation.Prcnt =sd(norm_counts) / mean(norm_counts) * 100
)
}
# Save stats data for GOI
for (i in seq_along(counts_data_list_goi_stats)) {
write.csv(
counts_data_list_goi_stats[[i]],
file = paste0(output_dir_normCounts, names(counts_data_list_goi_stats)[i], "_stats.csv"),
row.names = TRUE
)
}
# Boxplots on linear scale
b <- list()
for (i in seq_along(counts_data_list_goi)) {
b[[i]] <- ggplot(data = counts_data_list_goi[[i]],
aes(x = factor(counts_data_list_goi[[i]]$condition, levels = mixedsort(unique(counts_data_list_goi[[i]]$condition))),
y = counts_data_list_goi[[i]]$norm_counts)) +
geom_boxplot() +
geom_point() +
xlab("Genotype") +
ylab("Normalized Counts") +
scale_y_continuous() +
labs(title = paste0(names(counts_data_list_goi[i]), " DEP_normCounts")) +
theme(axis.text.x = element_text(angle = 90, hjust = 0.5, vjust = 0.5, size=rel(1)))
print(b[[i]])
ggsave(filename = paste0(
graph_dir_normCounts,
names(counts_data_list_goi)[[i]],
"_normCounts",
".pdf"
))
}
# Boxplots on linear scale
b <- list()
for (i in seq_along(counts_data_list_goi)) {
b[[i]] <- ggplot(data = counts_data_list_goi[[i]],
aes(x = counts_data_list_goi[[i]]$condition ,
y = counts_data_list_goi[[i]]$norm_counts)) +
geom_boxplot() +
geom_point() +
xlab("Genotype") +
ylab("Normalized Counts") +
scale_y_continuous() +
labs(title = paste0(names(counts_data_list_goi[i]), " DEP_normCounts")) +
theme(axis.text.x = element_text(angle = 90, hjust = 0.5, vjust = 0.5, size=rel(1)))
print(b[[i]])
ggsave(filename = paste0(
graph_dir_normCounts,
names(counts_data_list_goi)[[i]],
"_normCounts",
".pdf"
))
}
# Graphics termination
if(!is.null(dev.list())) dev.off()
if(!is.null(dev.list())) graphics.off()
})
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## user system elapsed
## 998.139 2.782 1097.893
comps_ls <- list()
for (h in seq_along(DEP_TMT_EDA_mv_ls)){
# Directories
dir.create(paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res"))
output_dir_res <- paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res/")
dir.create(paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res"))
graph_dir_res <- paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res/")
# Extract one
DEP_mv <- DEP_TMT_EDA_mv_ls[[h]]
# comps
comps_factor <- as.vector(factor(paste0(colData(DEP_mv)$condition)))
comps_df <- crossing(comp_num=comps_factor, comp_denom=comps_factor)
comps_df <- subset(comps_df, as.character(comps_df$comp_num) != as.character(comps_df$comp_denom))
# All comps
comps_df_all <- comps_df
comps_df_all <- cbind(comp_type="condition",comps_df_all)
comps_df_all <- cbind(comp_no=1:nrow(comps_df_all),comps_df_all)
comps_df_all$comp_name <- paste(comps_df_all$comp_num, "vs", comps_df_all$comp_denom, sep = "-")
comps_df_all$comp <- paste(comps_df_all$comp_num,comps_df_all$comp_denom, sep="-")
write.csv(comps_df_all, file=paste0(output_dir_res,"comps_all.csv"), col.names = T, row.names = F)
comps_all_subset <- comps_df_all[,2:4]
# Unique comps only (for only unique combo)
comps_df_unique <- comps_df[!duplicated(t(apply(comps_df, 1, sort))), ]
comps_df_unique <- cbind(comp_type="condition",comps_df_unique )
comps_df_unique <- cbind(comp_no=1:nrow(comps_df_unique),comps_df_unique)
comps_df_unique$comp_name <- paste(comps_df_unique$comp_num, "vs", comps_df_unique$comp_denom, sep = "-")
comps_df_unique$comp <- paste(comps_df_unique$comp_num,comps_df_unique$comp_denom, sep="-")
write.csv(comps_df_unique, file=paste0(output_dir_res,"comps_unique.csv"), col.names = T, row.names = F)
comps_unique_subset <- comps_df_unique[,2:4]
# Comp list to use (all comps)
comps <- comps_df_all
# List of comps
comps_ls[[names(DEP_TMT_EDA_mv_ls)[h]]] <- comps
}
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_all, file = paste0(output_dir_res,
## "comps_all.csv"), : attempt to set 'col.names' ignored
## Warning in write.csv(comps_df_unique, file = paste0(output_dir_res,
## "comps_unique.csv"), : attempt to set 'col.names' ignored
# Extract
# Results
# mibs_comp_list
DEP_TMT_EDA_limmaRes <- list()
for (h in seq_along(comps_ls)){
comps <- comps_ls[[h]]
# Directories
dir.create(paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res"))
output_dir_res <- paste0(output_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res/")
dir.create(paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res"))
graph_dir_res <- paste0(graph_dir,names(DEP_TMT_EDA_mv_ls)[[h]],"/","Limma_res/")
# Extract results
DEP_TMT_limmaRes <- list()
for (i in seq_along(comps$comp)){
DEP_TMT_limmaRes[[comps$comp[i]]] <-
mibs_comp_list[[paste0(comps$comp[i])]]
}
# save results
for (i in seq_along(DEP_TMT_limmaRes)) {
write.csv(
DEP_TMT_limmaRes[[i]],
file = paste0(output_dir_res,"comp_", i, "_", names(DEP_TMT_limmaRes)[i], ".csv"),
row.names = TRUE
)
}
DEP_TMT_EDA_limmaRes[[names(comps_ls)[h]]] <- DEP_TMT_limmaRes
}
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/C/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/C/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/CEv3/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/CEv3/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G12/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G12/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G8/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G8/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G5/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G5/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G1/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G1/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/E5/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/E5/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/E4/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/E4/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/TMT_samples_BL_Res/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/TMT_samples_BL_Res/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/TMT_samples_Res/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/TMT_samples_Res/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_mv_ls)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/TMT_samples_Par/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_mv_ls)[[h]], "/", :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/TMT_samples_Par/Limma_res' already exists
for(h in seq_along(DEP_TMT_EDA_limmaRes)){
comps_df_all <- comps_ls[[names(DEP_TMT_EDA_limmaRes)[h]]]
DEP_TMT_limmaRes <- DEP_TMT_EDA_limmaRes[[h]]
# Directories
dir.create(paste0(output_dir,names(DEP_TMT_EDA_limmaRes)[[h]],"/","Limma_res"))
output_dir_res <- paste0(output_dir,names(DEP_TMT_EDA_limmaRes)[[h]],"/","Limma_res/")
dir.create(paste0(graph_dir,names(DEP_TMT_EDA_limmaRes)[[h]],"/","Limma_res"))
graph_dir_res <- paste0(graph_dir,names(DEP_TMT_EDA_limmaRes)[[h]],"/","Limma_res/")
# Extract comps of interest
coi <- comps_df_all[grep("0",comps_df_all$comp_denom),]
comp_list_oi <- DEP_TMT_limmaRes[names(DEP_TMT_limmaRes) %in% coi$comp]
comp_list_oi <- lapply(comp_list_oi,data.frame)
# Label list of DF
for (i in seq_along(comp_list_oi)){
colnames(comp_list_oi[[i]]) <- paste0(colnames(comp_list_oi[[i]]),"_",names(comp_list_oi)[[i]])
comp_list_oi[[i]]$Gene_ID <- row.names(comp_list_oi[[i]])
}
# Left_join comps of interest to 1x spreadsheet
comp_list_oi_master <- Reduce(function(x, y) left_join(x, y, by="Gene_ID"), comp_list_oi)
row.names(comp_list_oi_master) <- comp_list_oi_master$Gene_ID
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)=="Gene_ID")]
comp_list_oi_master <- comp_list_oi_master[,-which(colnames(comp_list_oi_master)%in% paste0(grep("gene_",colnames(comp_list_oi_master), value = T)))]
# Subset to spreadsheets
comp_list_oi_padj <- comp_list_oi_master[,grep("adj.P.Val_", colnames(comp_list_oi_master))]
comp_list_oi_pvalue <- comp_list_oi_master[,grep("P.Value_", colnames(comp_list_oi_master))]
comp_list_oi_l2fc <- comp_list_oi_master[,grep("logFC_", colnames(comp_list_oi_master))]
# Save spreadsheets
write.csv(comp_list_oi_padj, file = paste0(output_dir_res,"All_coi_","padj",".csv"))
write.csv(comp_list_oi_pvalue, file = paste0(output_dir_res,"All_coi_","pvalue",".csv"))
write.csv(comp_list_oi_l2fc, file = paste0(output_dir_res,"All_coi_","l2fc",".csv"))
}
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/C/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/C/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/CEv3/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/CEv3/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G12/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G12/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G8/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G8/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G5/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G5/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/G1/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/G1/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/E5/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/E5/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/E4/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/E4/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/TMT_samples_BL_Res/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/TMT_samples_BL_Res/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/TMT_samples_Res/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/TMT_samples_Res/Limma_res' already exists
## Warning in dir.create(paste0(output_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Output/MIB-DYN/DEP_TMT_EDA/TMT_samples_Par/Limma_res' already exists
## Warning in dir.create(paste0(graph_dir, names(DEP_TMT_EDA_limmaRes)[[h]], :
## '/Data/Graphs/MIB-DYN/DEP_TMT_EDA/TMT_samples_Par/Limma_res' already exists
for(h in seq_along(DEP_TMT_EDA_limmaRes)){
# Extract one
subset_limmaRes <- DEP_TMT_EDA_limmaRes[[h]]
# Directory for volcano plots
dir.create(paste0(graph_dir,names(DEP_TMT_EDA_limmaRes)[h],"/","volcano"))
graph_dir_vol <- paste0(graph_dir,names(DEP_TMT_EDA_limmaRes)[h],"/","volcano/")
# Plot Volcano
volcano_plots_ls <- list()
for (i in seq_along(subset_limmaRes)){
plot(volcano_plots_ls[[names(subset_limmaRes)[i]]] <-
EnhancedVolcano(
subset_limmaRes[[i]],
lab=row.names(subset_limmaRes[[i]]),
x="logFC",
y="adj.P.Val",
title= paste0("MIBs ",names(subset_limmaRes)[[i]]),
pCutoff =qval,
FCcutoff =lfc,
pointSize = 2.0,
labSize = 3.0
))}
# Save Volcano
for (i in seq_along(volcano_plots_ls)){
ggsave(filename = paste0(graph_dir_vol,"comp_", i, "_", names(volcano_plots_ls)[[i]],".pdf"), plot=volcano_plots_ls[[i]])
}
# Graphics termination
if(!is.null(dev.list())) dev.off()
if(!is.null(dev.list())) graphics.off()
}
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 5 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
## Saving 7 x 7 in image
sessionInfo()
## R version 4.3.2 (2023-10-31)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 22.04.4 LTS
##
## Matrix products: default
## BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.20.so; LAPACK version 3.10.0
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## time zone: Etc/UTC
## tzcode source: system (glibc)
##
## attached base packages:
## [1] parallel grid stats4 stats graphics grDevices utils
## [8] datasets methods base
##
## other attached packages:
## [1] factoextra_1.0.7 cluster_2.1.8
## [3] gtools_3.9.5 limma_3.58.1
## [5] DEP_1.24.0 PCAtools_2.14.0
## [7] ggpubr_0.6.0 gprofiler2_0.2.3
## [9] vsn_3.70.0 ComplexHeatmap_2.18.0
## [11] UpSetR_1.4.0 msigdbr_7.5.1
## [13] GSVA_1.50.5 RSQLite_2.3.9
## [15] rmarkdown_2.29 gplots_3.2.0
## [17] EnhancedVolcano_1.20.0 ggrepel_0.9.6
## [19] org.Hs.eg.db_3.18.0 AnnotationDbi_1.64.1
## [21] BiocParallel_1.36.0 lubridate_1.9.4
## [23] forcats_1.0.0 stringr_1.5.1
## [25] purrr_1.0.4 readr_2.1.5
## [27] tidyr_1.3.1 tidyverse_2.0.0
## [29] pheatmap_1.0.12 RColorBrewer_1.1-3
## [31] colorRamps_2.3.4 cowplot_1.1.3
## [33] gridExtra_2.3 scales_1.3.0
## [35] ggplot2_3.5.1 DESeq2_1.42.1
## [37] SummarizedExperiment_1.32.0 MatrixGenerics_1.14.0
## [39] matrixStats_1.5.0 tximport_1.30.0
## [41] Biobase_2.62.0 dplyr_1.1.4
## [43] biomaRt_2.58.2 rtracklayer_1.62.0
## [45] GenomicRanges_1.54.1 GenomeInfoDb_1.38.8
## [47] IRanges_2.36.0 S4Vectors_0.40.2
## [49] BiocGenerics_0.48.1 tibble_3.2.1
##
## loaded via a namespace (and not attached):
## [1] ProtGenerics_1.34.0 bitops_1.0-9
## [3] httr_1.4.7 doParallel_1.0.17
## [5] tools_4.3.2 MSnbase_2.28.1
## [7] backports_1.5.0 DT_0.33
## [9] R6_2.6.1 HDF5Array_1.30.1
## [11] lazyeval_0.2.2 rhdf5filters_1.14.1
## [13] GetoptLong_1.0.5 withr_3.0.2
## [15] prettyunits_1.2.0 preprocessCore_1.64.0
## [17] textshaping_1.0.0 cli_3.6.4
## [19] sandwich_3.1-1 labeling_0.4.3
## [21] sass_0.4.9 mvtnorm_1.3-3
## [23] systemfonts_1.2.1 Rsamtools_2.18.0
## [25] rstudioapi_0.17.1 impute_1.76.0
## [27] generics_0.1.3 shape_1.4.6.1
## [29] BiocIO_1.12.0 car_3.1-3
## [31] Matrix_1.6-5 MALDIquant_1.22.3
## [33] imputeLCMD_2.1 abind_1.4-8
## [35] lifecycle_1.0.4 yaml_2.3.10
## [37] carData_3.0-5 rhdf5_2.46.1
## [39] SparseArray_1.2.4 BiocFileCache_2.10.2
## [41] blob_1.2.4 promises_1.3.2
## [43] dqrng_0.4.1 crayon_1.5.3
## [45] shinydashboard_0.7.2 lattice_0.22-6
## [47] beachmat_2.18.1 annotate_1.80.0
## [49] mzR_2.36.0 KEGGREST_1.42.0
## [51] pillar_1.10.1 knitr_1.49
## [53] rjson_0.2.23 codetools_0.2-20
## [55] glue_1.8.0 pcaMethods_1.94.0
## [57] data.table_1.16.4 vctrs_0.6.5
## [59] png_0.1-8 gtable_0.3.6
## [61] assertthat_0.2.1 cachem_1.1.0
## [63] xfun_0.51 mime_0.12
## [65] S4Arrays_1.2.1 ncdf4_1.23
## [67] SingleCellExperiment_1.24.0 iterators_1.0.14
## [69] gmm_1.8 statmod_1.5.0
## [71] bit64_4.6.0-1 progress_1.2.3
## [73] filelock_1.0.3 bslib_0.9.0
## [75] affyio_1.72.0 tmvtnorm_1.6
## [77] irlba_2.3.5.1 KernSmooth_2.23-26
## [79] colorspace_2.1-1 DBI_1.2.3
## [81] tidyselect_1.2.1 bit_4.5.0.1
## [83] compiler_4.3.2 curl_6.2.0
## [85] graph_1.80.0 xml2_1.3.6
## [87] DelayedArray_0.28.0 plotly_4.10.4
## [89] caTools_1.18.3 hexbin_1.28.5
## [91] affy_1.80.0 rappdirs_0.3.3
## [93] digest_0.6.37 XVector_0.42.0
## [95] htmltools_0.5.8.1 pkgconfig_2.0.3
## [97] sparseMatrixStats_1.14.0 dbplyr_2.5.0
## [99] fastmap_1.2.0 rlang_1.1.5
## [101] GlobalOptions_0.1.2 htmlwidgets_1.6.4
## [103] shiny_1.10.0 DelayedMatrixStats_1.24.0
## [105] farver_2.1.2 jquerylib_0.1.4
## [107] zoo_1.8-13 jsonlite_1.9.0
## [109] mzID_1.40.0 BiocSingular_1.18.0
## [111] RCurl_1.98-1.16 magrittr_2.0.3
## [113] Formula_1.2-5 GenomeInfoDbData_1.2.11
## [115] Rhdf5lib_1.24.2 munsell_0.5.1
## [117] Rcpp_1.0.14 babelgene_22.9
## [119] MsCoreUtils_1.14.1 stringi_1.8.4
## [121] zlibbioc_1.48.2 MASS_7.3-60
## [123] plyr_1.8.9 Biostrings_2.70.3
## [125] hms_1.1.3 circlize_0.4.16
## [127] locfit_1.5-9.11 ggsignif_0.6.4
## [129] reshape2_1.4.4 ScaledMatrix_1.10.0
## [131] XML_3.99-0.18 evaluate_1.0.3
## [133] BiocManager_1.30.25 httpuv_1.6.15
## [135] tzdb_0.4.0 foreach_1.5.2
## [137] clue_0.3-66 norm_1.0-11.1
## [139] rsvd_1.0.5 broom_1.0.7
## [141] xtable_1.8-4 restfulr_0.0.15
## [143] later_1.4.1 rstatix_0.7.2
## [145] ragg_1.3.3 viridisLite_0.4.2
## [147] memoise_2.0.1 GenomicAlignments_1.38.2
## [149] timechange_0.3.0 GSEABase_1.64.0
proc.time() - ptm
## user system elapsed
## 2398.887 8.533 2645.174